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SCAR PRIMERS AND A KIT FOR THE AUTHENTICATION OF UNANI DRUG SENNA (CASSIA ACUTIFOLIA amp; CASSIA ANGUSTIFOLIA) FROM ITS ADULTERANT (CASSIA TORA amp; CASSIA SOPHERA)'.
SCAR PRIMERS AND A KIT FOR THE AUTHENTICATION OF UNANI DRUG SENNA (CASSIA ACUTIFOLIA amp; CASSIA ANGUSTIFOLIA) FROM ITS ADULTERANT (CASSIA TORA amp; CASSIA SOPHERA)'.
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机译：从其促进剂（CASSIA TORA和CASSIA SOPHERA）中提取伤疤的小品和用于认证UNANI DRUG SENNA（卡西娅·索蒂斯和卡西娅·安古斯迪菲利亚）的工具”。
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摘要
This invention relates to SCAR Primers and a Kit for the Authentication of Unani drug Senna (Cassia acutifolia & Cassia angustifolia ) from its adulterant (Cassia tora & Cassia sophera), 1(a) Genuine drug ,Senna (Cassia acutifolia) 5"-GCTAGATCACTACAACAACACAACA-3", 5" -TTTTGGGATGGGGA ATAACA-3", and 1(b) " (Cassia angustifolia), 5"CCAATTTACGCCCCATACAG3", 5GTATCGTACAGCGTAGTGCTACAGAG-3"and its adulterant 1(c) Cassia tora, 5"-CGA CAATGAGAAAATCCAACC-3", 5"TGATTGAAACACCCCATTAGC-3", and 1(d) Cassia sophera, 5"-GTTCTATAAGAATTGGTGCAAGTCC-3", 5"AGGAA CAGCTATGACCATGATTAC-3", and a kit for authentication of genuine-drug, which comprises: kit, an oligonucleotide primer set genuine drug Cassia acutifolia that comprises a forward oligonucleotide primer having the nucleotide sequence set forth in SEQ ID NO:35 (5"-GCTAGATCACTACAACAACACAACA-3"), and a reverse oligonucleotide primer having the nucleotide sequence set forth in SEQ ID NO:36 (5"-GCTAGATCACTACAACAACACAACA-3"), an oligonucleotide primer set genuine drug Cassia angustifolia that comprises a forward oligonucleotide primer having the nucleotide sequence set forth in SEQ ID NO:37 (5-CCAATTTACGCCCCATACAG3"), and a reverse oligonucleotide primer having the nucleotide sequence set forth in SEQ ID NO:38 (5"GTATCGTACAGCGTAGTGCTACAGAG-3"), (c) an oligonucleotide primer set for the adulterant Cassia tora that comprises a forward oligonucleotide primer having the nucleotide sequence set forth in SEQ ID NO:39(5"-CGACAATGAGAAAATCCAAC C-3") and a reverse oligonucleotide primer having the nucleotide sequence set forth in SEQ ID NO:40 (5"TGATTGAAACACCCCATTAGC-3"), (d) an oligonucleotide primer set for the adulterant Cassia sophera that comprises a forward oligonucleotide primer having the nucleotide sequence set forth in SEQ ID NO:41(5"-GTTCTATAGAATTGGT TGGTGCAAGTCC-3") and a reverse oligonucleotide primer having the nucleotide sequence set forth in SEQ ID NO:42(5"AGGAACAGCTATGACCATGATTAC-3").
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机译：本发明涉及SCAR引物和用于从其掺假品（决明子（Cassia tora）和决明子）中鉴定乌纳尼药物番泻叶（决明子和决明子），1（a）真药番泻叶（决明子）5” -GCTAGATCACTACAACAACACAACACA的SCAR引物和试剂盒-3英寸，5英寸-TTTTGGGATGGGGA ATAACA-3英寸和1（b）英寸（决明子），5英寸CCAATTTACGCCCCATACAG3英寸，5GTATCGTACAGCGTAGTGCTACAGAG-3英寸及其掺假的1（c决明子），5英寸-CGA CAATGAGAAAATCCAACC ”，“ 5” TGATTGAAACACCCCATTAGC-3”和1（d）决明子，5” -GTTCTATAAGAATTGGTGCAAGTCC-3”，5” AGGAA CAGCTATGACCATGATTAC-3”和用于正品鉴定的试剂盒，包括：试剂盒，寡核苷酸引物组真药决明子，其包含具有SEQ ID NO：35所示核苷酸序列的正向寡核苷酸引物（5“ -GCTAGATCACTACAACAACACACAACA-3”），和具有SEQ ID NO：5所示核苷酸序列的反向寡核苷酸引物36（5“ -GCTAGATCACTACAACAACACAACA-3”），寡核苷酸引物组的一种新药决明子，其包含具有SEQ ID NO：37（5-CCAATTTACGCCCCATACAG3“）的核苷酸序列的正向寡核苷酸引物和具有SEQ ID NO：38（5” GTATCGTACAGCGCGTAGTGCTACAGAG -3”），（c）用于掺假决明子的寡核苷酸引物组，其包含具有SEQ ID NO：39（5” -CGACAATGAGAAAATCCAAC C-3”）所示核苷酸序列的正向寡核苷酸引物和反向寡核苷酸引物（d）掺假决明子的寡核苷酸引物组，其具有SEQ ID NO：40（5“ TGATTGAAACACCCCATTAGC-3”）所示的核苷酸序列，该寡核苷酸引物组包含具有SEQ ID NO：4所示核苷酸序列的正向寡核苷酸引物41（5″ -GTTCTATAGAATTGGT TGGTGCAAGTCC-3″）和具有SEQ ID NO：42（5″ AGGAACAGCTATGACCATGATTAC-3″）所示核苷酸序列的反向寡核苷酸引物。
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