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Methods for making langerhan cells or dendritic cells and for differentiating monocytes, cell model, frozen cd14 + monocytes, use of at least one cell model and / or at least one tissue model, and methods for producing a tissue model , and a reconstructed skin or a reconstructed mucosa
Methods for making langerhan cells or dendritic cells and for differentiating monocytes, cell model, frozen cd14 + monocytes, use of at least one cell model and / or at least one tissue model, and methods for producing a tissue model , and a reconstructed skin or a reconstructed mucosa
The present invention relates to a method for preparing Langerhans cells (LC), or dermal/interstitial dendritic cells (IDC) or LCs and IDCs from CD 14+ monocytes stemming from peripheral circulatory blood of a living being, and notably of a human being, comprising differentiation of CD 14+ monocytes into LCs, or into IDCs, or into LCs and IDCs by putting the CD 14+ monocytes in the presence of a cell environment comprising epithelial cells, such as keratinocytes, and/or mesenchymatous cells such as dermal fibroblasts. The present invention also relates to cell or tissue models comprising at least LCs and/or IDCs, and optionally macrophages and endothelial cells, able to be obtained according to this method, and to their uses.
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