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Fertility restorer gene and fertility restoration method for CW-type male sterile cytoplasm of rice
Fertility restorer gene and fertility restoration method for CW-type male sterile cytoplasm of rice
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机译:水稻CW型雄性不育细胞质的育性恢复基因和育性恢复方法
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摘要
Mainly provided is a technique for directly identifying the genotype at locus Rf17 based on the specific base sequence data thereof. Also provided is a technique for artificially constructing a fertility-restored line. A method of restoring the fertility of CW-type cytoplasmic male sterile rice by inhibiting or reducing the expression of a gene comprising the base sequence represented by SEQ ID NO:2 in the above-described rice, and a method for determining the presence or absence of gene Rf17, which is a fertility restorer gene for CW-type cytoplasmic male sterility, comprising identifying a single nucleotide polymorphism (SNP) in the base at the 1812 position of the base sequence represented by SEQ ID NO:1 in the rice to be examined.
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机译:主要提供一种基于其特定碱基序列数据直接鉴定基因座Rf17处的基因型的技术。还提供了一种人为地构建生育力恢复系的技术。通过抑制或减少上述水稻中包含SEQ ID NO:2所示碱基序列的基因的表达来恢复CW型细胞质雄性不育水稻的育性的方法,以及确定存在与否的方法Rf17的基因,它是CW型细胞质雄性不育的育性恢复基因,包括在待鉴定的水稻中鉴定SEQ ID NO:1表示的碱基序列的1812位置的碱基中的单核苷酸多态性(SNP)。检查。
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