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Quantitative PCR-based compositions and methods for the diagnosis of invasive pulmonary aspergillosis
Quantitative PCR-based compositions and methods for the diagnosis of invasive pulmonary aspergillosis
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机译:基于定量PCR的侵袭性肺曲霉病诊断组合物和方法
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摘要
Provided are quantitative PCR-based compositions and methods for the diagnosis of invasive pulmonary aspergillosis (IPA) in a patient sample, such as bronchoalveolar lavage (BAL) fluid. The methods presented herein involve isolating a patient sample, optionally extracting DNA from the sample, carrying out a quantitative PCR (qPCR) reaction on the sample to generate an amplicon that includes a region of an Aspergillus spp. ribosomal RNA (rRNA) gene, and detecting the PCR amplicon. The present disclosure also provides primers and primer sets for specifically detecting an Aspergillus spp. fungal pathogen in the presence of human ribosomal DNA (rDNA).
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