首页> 外国专利> Mutated nucleotide sequences of batroxobin, mutated α factor secretion signal sequence and processes for preparing batroxobin using the same

Mutated nucleotide sequences of batroxobin, mutated α factor secretion signal sequence and processes for preparing batroxobin using the same

机译:巴曲酶的核苷酸序列突变,α因子分泌信号突变的序列及其制备巴曲酶的方法

摘要

The present invention relates to a batroxobin-encoding nucleotide sequence and/or a mutated α-factor secretion signal sequence, and a vector and a transformant using the same. The batroxobin-encoding nucleotide sequence of this invention exhibits an excellent expression efficiency in yeast, particular Pichia pastoris and the recombinant batroxobin is obtained at 4-13 fold higher yield than natural-occurring batroxobin-encoding sequences. The protein expression system which uses the batroxobin-encoding nucleotide sequence as well as mutated α-factor secretion signal peptide sequence of this invention obtains the recombinant batroxobin at about 20-fold higher yield than natural-occurring batroxobin-encoding sequences. In addition, the recombinant batroxobin prepared using the sequence of this invention has a significantly plausible activity and stability compared with natural-occurring batroxobin.
机译:本发明涉及编码巴曲酶的核苷酸序列和/或突变的α因子分泌信号序列,以及使用它们的载体和转化体。本发明的编码巴曲酶的核苷酸序列在酵母特别是巴斯德毕赤酵母中显示出优异的表达效率,并且重组巴曲酶的产率比天然的巴曲酶编码序列高4-13倍。使用本发明的编码巴曲酶的核苷酸序列以及突变的α因子分泌信号肽序列的蛋白质表达系统以比天然巴曲酶编码的序列高约20倍的产率获得重组巴曲酶。另外,与天然存在的巴曲酶相比,使用本发明的序列制备的重组巴曲酶具有明显合理的活性和稳定性。

著录项

相似文献

  • 专利
  • 外文文献
  • 中文文献
获取专利

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号