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Cell mass state discrimination technique, image processing program and image processing apparatus using the technique, and cell mass production method

机译:细胞质量状态鉴别技术,使用该技术的图像处理程序和图像处理装置以及细胞大量生产方法

摘要

Provided is a means for discriminating and outputting a state of multi-layering of cell clusters from a time-series image. The image processing program (GP) includes a step of acquiring a time-series image (S10), a step of extracting a cell cluster from each acquired image (S20), and a spatial luminance value of a small region in the extracted cell cluster Calculating the distribution of the luminance and temporal change in luminance value for the entire cell mass (S50), and determining the multi-layered state of the cell mass based on the spatial distribution and time-series change of the calculated luminance value (S70) and a step (S80) of outputting a discrimination result, and configured to discriminate and output the state of the cell mass multi-layer from the time-series image of the cell mass.
机译:提供了一种用于从时序图像中区分和输出细胞簇的多层状态的手段。图像处理程序(GP)包括获取时间序列图像的步骤(S10),从每个获取的图像中提取单元簇的步骤(S20)以及所提取的单元簇中的小区域的空间亮度值。计算整个单元质量的亮度分布和亮度值的时间变化(S50),并基于计算出的亮度值的空间分布和时间序列变化确定单元质量的多层状态(S70)步骤(S80),其输出判别结果,并从细胞团的时间序列图像中判别并输出细胞团多层的状态。

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