Providing a prozone phenomenon detection method can detect the occurrence of the prozone phenomenon easily even with a sample analysis device prior to efficiently perform inspection using the immunochromatography method. Using the sample analysis tool that includes a substance that specifically binds to the analyte of interest in a sample, wherein the sample analysis tool, the porous base material, from upstream to downstream in the moving direction of the sample, the sample supply unit, a reagent unit and a sample analysis device detection unit is disposed, and a labeling substance, wherein the reagent portion, specifically binds to said analyte, wherein the detection unit, specifically binds to said analyte The analyte is detected by including the constant substances that, in the detector to detect the label of the labeled substance, a complex of the immobilized substance of the analyte, and the labeled substance , the following method A and is characterized in that it comprises at least one of B method. In the detector, Method B method were plotted and the detection result in the movement direction of the sample, to detect the occurrence of the prozone phenomenon from the position of the peak of the plot:: A method at the detector, the detection of the label how to implement two or more times at different times, and detects the occurrence of prozone phenomena magnitude relation of the detection results of two or more obtained
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