Cryosurgical instrument for separating tissue samples from surrounding tissues of the biological tissue to be processed

• 1. 用于将组织样品与待治疗的生物组织的周围组织分离的冷冻外科器械 [P] . 中国专利： CN101563039B . 2012.01.04
• 2. 用于将组织样品与待治疗的生物组织的周围组织分离的冷冻外科器械 [P] . 中国专利： CN101563039A . 2009-10-21
• 3. Cryosurgical instrument for separating tissue samples from surrounding tissues of the biological tissue to be processed [P] . 日本专利： JP2010512873A . 2010-04-30

  机译： 用于从要处理的生物组织的周围组织中分离组织样本的冷冻外科器械

• 4. Cryosurgical instrument for separating a tissue sample from surrounding tissue of a biological tissue that is to be treated [P] . 美国专利： US8308718B2 . 2012-11-13

  机译： 用于从要治疗的生物组织的周围组织中分离组织样品的冷冻外科器械

• 5. CRYOSURGICAL INSTRUMENT FOR SEPARATING A TISSUE SAMPLE FROM SURROUNDING TISSUE OF A BIOLOGICAL TISSUE THAT IS TO BE TREATED [P] . PL2114276T3 . 2012-10-31

  机译： 用于将组织样本与要处理的生物组织的组织分离的冷冻外科器械

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• 3. Correlation of reactive oxygen species contents in myocardial tissue and lung tissue with tissue injury and apoptosis in the rat models with sepsis [J] . Wan-Ping Liu 海南医科大学学报(英文版) . 2018,第008期
• 4. Usefulness of Color Coding Resected Samples from a Pancreaticoduodenectomy with Tissue Marking Dyes for a Detailed Examination of Surgical Margin Surrounding the Uncinate Process of the Pancreas [J] . Satoshi Mizutani, Hideyuki Suzuki, Takayuki Aimoto, Journal of Nippon Medical School . 2017,第1期
• 5. Thermal management in a biological tissue in order to destroy tissue under local heating process [J] . Pouya Barnoon, Farnoush Bakhshandehfard Case Studies in Thermal Engineering . 2020,第a期
• 6. A Useful Instrument to Protect the Surrounding Tissues in Craniofacial Surgery: Sterile Metal Tablespoon [J] . Ceran Candemir, Keskin Durdane, Cicek Cagla Amutkan, The Journal of craniofacial surgery . 2015,第6期
• 7. ANIMATION OF CRYOSURGICAL PROCESS OF BIOLOGICAL TISSUES DURING TUMOR TREATMENT [C] . Wei RAO, Z.Q.SUN, L.GUI, . 2007
• 8. Development of an Instrument for the Chemical and Isotopic Analysis of Sub-nanomole Quantities of Gas from Individual Fluid Inclusions, Microfossils and Biological Tissues Using Mass Spectrometry [D] . Emmons, Matthew . 2019
• 9. Impact of optical tissue clearing on the Brillouin signal from biological tissue samples [O] . Rafael J. Jiménez Riobóo, Manuel Desco, María Victoria Gómez-Gaviro 2019
• 10. Impact of optical tissue clearing on the Brillouin signal from biological tissue samples [O] . Rafael J. Jiménez Riobóo, Manuel Desco, María Victoria Gómez-Gaviro 2019
• 11. Radiation Preservation of Biological Tissues with Special Emphasis on Immunological Alterations. Part of a Coordinated Program on Radiosterilization of Medical Products and Biological Tissues. Final Report for the Period 15 March 1973--30 September 1976 [R] . Feher, I. 1977

• 1. BACKGROUND： Vibrio vulnifi cus inside the body could activate the NF-!B signaling pathwayand initiate the inflammatory cascade. The lung is one of the earliest organs affected by sepsisassociated with acute lung injury. High mobility group protein B1 （HMGB1） is an important late-actingpro-infl ammatory cytokine involving in the pathophysiology of sepsis. It is also involved in the injuryprocess in the lung, liver and intestine. There has been no report on the involvement of HMGB1 inVibrio vulnifi cus sepsis-induced lung injury.METHODS： Sixty rats were randomly divided into a normal control group （group A, n=10） anda Vibrio vulnificus sepsis group （group B, n=50）. Sepsis was induced in the rats by subcutaneousinjection of Vibrio vulnificus （concentration 6×108 cfu/mL, volume 0.1 mL/100g）） into the left lowerlimbs. The rats in group B were sacrifi ced separately 1, 6, 12, 24, and 48 hours after the infection.Their lungs were stored as specimens, lung water content was measured, and lung pathology wasobserved under a light microscope. The expressions of the HMGB1 gene and protein in the lungswere detected by RT-PCR and Western blot. Data were analyzed with one-way analysis of variance（ANOVA） and the LSD method for pair-wise comparison between the two groups. P〈0.05 wasconsidered statistically signifi cant.RESULTS： Compared to group A （0.652±0.177）, HMGB1 mRNA expression in the lungs ofgroup B was signifi cantly higher at 0 hour （1.161±0.358, P=0.013）, 24 hours （1.679±0.235, P=0.000）,and 48 hours （1.258±0.274, P=0.004） （P〈0.05）, and peaked at 24 hours. Compared to group A（0.594±0.190）, HMGB1 protein expression at 6 hours （1.408±0.567, P=0.026） after infection wassignificantly increased （P〈0. 05）, and peaked at 24 hours （2.415±1.064, P=0.000） after infection.Compared to group A （0.699±0.054）, lung water content was significantly increased at 6 hours（0.759±0.030, P=0.001）,12 hours （0.767±0.023, P=0.000）, 24 hours （0.771±0.043, P=0.000） and 48hours （0.789±0.137, P=0.000） after infection （P〈0.05）. Compared to group A, pathological changesat 12 hours in group B indicate marked pulmonary vascular congestion, interstitial edema andinfl ammatory infi ltration. Alveolar cavity collapse and boundaries of the alveolar septum could not beclearly identifi ed.CONCLUSION： Vibrio vulnifi cus sepsis can lead to injury in rat lungs, and increased HMGB1expression in lung tissue may be one of the mechanisms for injury from Vibrio vulnifi cus sepsis. [J] . Qiao-meng Qiu ,Zhong-wang Li ,Lu-ming Tang . 世界急诊医学杂志(英文) . 2011,第4期
• 2. Effect of sample treatment on determination of arsenic （Ⅲ） and arsenic （Ⅴ） in aqueous and tissue samples by hydride generation atomic absorption spectrometry [J] . Jamileh Salar-Amoli ,Tahereh Ali-Esfahani ,Jalal Hassan . 化学与化工 . 2009,第006期
• 3. 近红外光谱法用于子宫内膜癌组织诊断中光谱预处理研究 [C] . 张卓勇 . 全国第四届近红外光谱学术会议 . 2012
• 4. 促性腺激素释放激素激动剂预处理在兔卵巢组织玻璃化冷冻与自体移植中作用的实验研究 [A] . 冯翠娥 . 2010