首页> 外国专利> Genotyping Legionella pneumophila comprises coamplifying variable-number-tandem-repeats loci in medium having L. pneumophila DNA sample, analyzing amplicon and assigning numerical multiple locus variable number tandem repeat analysis code

Genotyping Legionella pneumophila comprises coamplifying variable-number-tandem-repeats loci in medium having L. pneumophila DNA sample, analyzing amplicon and assigning numerical multiple locus variable number tandem repeat analysis code

机译：对肺炎军团菌进行基因分型包括在具有肺炎链球菌DNA样品的培养基中共扩增可变数串联重复基因座，分析扩增子并分配数字多基因座可变数目串联重复分析代码

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摘要

Method for genotyping Legionella pneumophilaby multiple-locus variable number tandem repeat analysis (MLVA) comprises: (i) carrying out a coamplification of at least ten variable-number-tandem-repeats (VNTR) loci by multiplex PCR in a reaction medium comprising a DNA sample of Legionella pneumophilato form many amplicons for each of the ten VNTR loci; (ii) analyzing the amplicons by capillary electrophoresis to obtain an electrophoretic profile of the sample; and (iii) assigning a numerical MLVA code to the electrophoretic profile. An independent claim is included for kit for genotyping Legionella pneumophilacomprising: the reaction medium containing at least eight labeled sense primers and at least eight antisense primers, forming at least eight pairs of sense/antisense primers to each of which represents a specific VNTR locus and different for each of the eight primer pairs, each of the eight sense primers hybridizing upstream of the respective locus and each of the eight anti-sense primers hybridizing downstream of its respective locus; amplification mix; and optionally a positive control and a false negative control, so validate the result and get rid of positive-false or negative-false.

机译：通过多基因座可变数目串联重复重复分析（MLVA）对肺炎军团菌进行基因分型的方法包括：（i）通过多重PCR在包含DNA的反应介质中对至少十个可变数目串联重复（VNTR）基因座进行共扩增肺炎军团菌的样本为十个VNTR基因座的每一个形成许多扩增子; （ii）通过毛细管电泳分析扩增子以获得样品的电泳图谱; （iii）将数字MLVA代码分配给电泳图谱。包含针对肺炎军团菌基因型的试剂盒的独立权利要求包括：反应培养基包含至少八种标记的有义引物和至少八种反义引物，形成至少八对有义/反义引物，每对代表特定的VNTR基因座，不同对于八个引物对中的每个，八个有义引物中的每个在相应基因座的上游杂交，八个反义引物中的每个在其各自基因座的下游杂交;放大混合;以及可选的阳性对照和假阴性对照，因此请验证结果并摆脱肯定错误或否定错误。

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公开/公告号FR2960552A1

专利类型
公开/公告日2011-12-02

原文格式PDF
申请/专利权人 CENTRE EUROPEEN DEXPERTISE ET DE RECHERCHE SUR LES AGENTS MICROBIENS - CEERAM;CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (CNRS);
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申请/专利号FR20100054237
发明设计人 LEBEAU BENOIT;LOISY-HAMON FABIENNE;SOBRAL DANIEL;POURCEL CHRISTINE;
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申请日2010-06-01
分类号C12Q1/68;C12Q1/04;G01N33/52;G06F19;
国家 FR
入库时间 2022-08-21 17:04:14

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