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In vitro method for evaluating quality of cartilage mass, comprises dissolving cartilage mass sample, separating isolated cartilage cells and proliferating, and determining proliferation ability of cells based on cell population size
In vitro method for evaluating quality of cartilage mass, comprises dissolving cartilage mass sample, separating isolated cartilage cells and proliferating, and determining proliferation ability of cells based on cell population size
In vitro method for evaluating the quality of cartilage mass, comprises (a) dissolving a sample of cartilage mass to obtain isolated cartilage cells; (b) separating isolated cartilage cells obtained in the step (a) and proliferating during a specified period, such that the proliferation of individual cells is recognized through the formation of cell populations; and (c) determining the proliferation ability of cells based on the size of the cell populations. An independent claim is also included for producing a cartilage transplants, comprising (i) dissolving a sample of cartilage mass to obtain isolated cartilage cells in vitro, (ii) separating isolated cartilage cells obtained in the step (i) and proliferating during a specified period, such that the proliferation of individual cells is recognized through the formation of cell populations, (iii) providing the cells with the best proliferation ability and applying to a three-dimensional resorbable frame, and (iv) culturing the cells on the resorbable frame until the transplant has the desired size and cell density.
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