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首页> 外国专利> DIAGNOSTIC TECHNIQUE FOR BLADDER CANCER BY CANCER-SPECIFIC MARKER TEDP1 (VERSIONS) AND KIT FOR IMPLEMENTING IT

DIAGNOSTIC TECHNIQUE FOR BLADDER CANCER BY CANCER-SPECIFIC MARKER TEDP1 (VERSIONS) AND KIT FOR IMPLEMENTING IT

机译：癌症专用标记TEDP1（版本）和实施套件的膀胱癌诊断技术

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FIELD: medicine.;SUBSTANCE: biomaterial preparation is RNA recovery, cDNA synthesis on the RNA matrix, control of the TFDP1 cDNA concentration by a control gene, quantitative PCR-amplification of a TFDP1 gene fragment. These procedures are followed by evaluation of the amplified TFDP1 DNA fragment for a biomaterial sample. If the TFDP1 gene cDNA concentration in physiological fluids exceeds 1.5% of the ACTB beta-actin gene cDNA concentration, the presence of transitional cell bladder cancer is diagnosed. A kit for implementing the CR-based technique includes two primers with a certain sequence at molar ratio 1:1. A version technique provides the ELISA-based diagnosing. Patient's urine and blood are sampled to recover a mixture of protein components of urine and blood that is followed by ELISA with monoclonal antibodies and/or polyclonal antibodies and/or their fragments to TFDP1 recombinant protein and/or its unique fragments more than 8 amino acids long. Bladder cancer is diagnosed if observing the TFDP1 protein concentration in the analysed samples exceeding the 5-fold TFDP1 protein concentration in the reference.;EFFECT: technique enables high-reliability diagnosing of bladder cancer, including at the early stage of progressing neoplastic aberration.;3 cl, 4 tbl, 4 dwg
机译：领域：医学;物质：生物材料的制备是RNA的回收，在RNA基质上的cDNA合成，通过控制基因控制TFDP1 cDNA浓度，定量PCR扩增TFDP1基因片段。这些步骤之后，评估生物材料样品的扩增的TFDP1 DNA片段。如果生理液中的TFDP1基因cDNA浓度超过ACTBβ-肌动蛋白基因cDNA浓度的1.5％，则表明存在移行性膀胱癌。用于实施基于CR的技术的试剂盒包括两个具有一定摩尔比1：1的序列的引物。版本技术提供了基于ELISA的诊断。对患者的尿液和血液进行采样以回收尿液和血液中蛋白质成分的混合物，然后通过ELISA与单克隆抗体和/或多克隆抗体和/或其针对TFDP1重组蛋白的片段和/或其独特的超过8个氨基酸的片段进行ELISA长。如果观察样品中的TFDP1蛋白浓度超过参考值的5倍TFDP1蛋白浓度，则诊断为膀胱癌。效果：该技术可实现膀胱癌的高可靠性诊断，包括在进行肿瘤畸变的早期阶段。 3厘升，4汤匙，4载重吨

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