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METHOD OF DETERMINING DEGREE OF EPIDEMIC HAZARD OF PATHOGENIC AND POTENTIALLY PATHOGENIC BACTERIA ISOLATED FROM WATER FOR VARIOUS USES
METHOD OF DETERMINING DEGREE OF EPIDEMIC HAZARD OF PATHOGENIC AND POTENTIALLY PATHOGENIC BACTERIA ISOLATED FROM WATER FOR VARIOUS USES
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机译：测定各种用途的水中分离的致病性和潜在性致病菌流行病危害程度的方法
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FIELD: chemistry.;SUBSTANCE: adhesive activity index (A), invasive activity index (I) and toxic activity index (T) of pathogenic bacteria is determined on continuous cells of a tissue culture of buffalo green monkey (BGM) kidney cells. A dose containing 107 colonies of bacterial cells is added to the culture monolayer of BGM cells and held at 37°C for 24 hours. The monolayer cells are washed 8 times with 10 ml of a physiological solution at pH 7.2. Index A is determined using a formula after first visually counting in Gorjaev's chamber living and dead cells stuck with bacterial growth and coloured by 0.1% trypan blue solution. Index (I) is determined using a formula after first replacing the culture medium in vials with a medium containing an antibiotic with concentration 50 mcg/ml and incubated in a temperature-controlled cabinet for 1 hour at 37°C. BGM cells are washed from bacteria 8 times with 10 ml of a physiological solution and lysed with 0.1% Triton X-100. Seeding of 0.1 ml is made from the obtained suspension based on the required cultivations per cup with Endo medium. Incubation is carried for 24 hours in a temperature-controlled cabinet at 37°C and the number of grown bacterial cells is counted. Index (T) is determined using a formula after first incubating bacteria in the BGM cell monolayer for 1 day. The suspension is centrifuged and filtered through membrane filters with pore diameter 0.22 mcm. The obtained substrate is put into a pure monolayer of continuous cells and the number of living and dead BGM cells is counted after one day.;EFFECT: invention enables to determine the degree of epidemic hazard of pathogenic bacteria isolated from water.;3 tbl, 4 ex
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机译：领域：化学;物质：在水牛绿猴（BGM）肾细胞的组织培养的连续细胞上测定病原菌的黏附活性指数（A），侵袭活性指数（I）和毒性活性指数（T）。将含有10 7 菌落的剂量添加到BGM细胞的培养单层中，并在37℃下保持24小时。将单层细胞用10 ml pH 7.2的生理溶液洗涤8次。在目视计数戈尔耶夫氏室中被细菌滋生并被0.1％台盼蓝溶液染色的活细胞和死细胞后，使用公式确定指数A。在首先用含有浓度为50 mcg / ml的抗生素的培养基替换小瓶中的培养基并在温度控制柜中于37℃下孵育1小时后，使用公式确定指数（I）。用10 ml生理溶液从细菌中将BGM细胞洗净8次，并用0.1％Triton X-100裂解。基于所需的每杯Endo培养基培养物，从获得的悬浮液中播种0.1 ml。在温度控制柜中于37°C孵育24小时，并计算细菌的生长数量。在将细菌首先在BGM细胞单层中孵育1天后，使用公式确定指标（T）。将悬浮液离心并通过孔径为0.22mcm的膜滤器过滤。将获得的底物置于纯的连续细胞单层中，并在一天后对活和死的BGM细胞进行计数。效果：本发明能够确定从水中分离出的致病细菌的流行病危害程度。3 tbl， 4前
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