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METHOD OF COLOUR nucleolar organizer in histological preparations and Pap smear

机译：组织学制备和巴氏涂片中彩色核仁组织者的方法

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FIELD: medicine.;SUBSTANCE: paraffin sections are prepared, fixed, coated with a colloidal developer prepared by mixing 2% aqueous gelatin in 1% formic acid and 50% aqueous AgNO3 in equal proportions, incubated for 30-60 minutes, washed and contrasted. The material is fixed in 10% neutral buffered formalin (pH 7.4) for 24 hours. The material is finished in ascending alcohols, encapsulated in paraffin to prepare the sections which are prepared in 2% formic acid in 96% ethanol for 20 minutes, dehydrated in 2 portions of 96%; the sections are prepared in 0.1% NaOH for 2 min 30 sec. Then they are dried. One drop of 100% AgNO3 is layered on the preparation. It is followed by incubation in a thermostat at 60°C for 1 min 40 sec in a humid chamber, and coating with one drop of 40% formaldehyde and colloidal developer. It is incubated for 20-50 seconds in the thermostat at the same temperature. The sections are washed in 4 portions of distilled water. The sections are processed with acetate buffer pH 2.4 for 1 minute; the preparations are contrasted with 0.2% aqueous methyl green for 10-15 seconds, purified in chloroform, differentiated for 2-3 minutes in n-butyl alcohol, processed in toluene and encapsulated in polystyrene.;EFFECT: higher quality of detection and evaluation of nucleolar organisers.;1 ex, 2 dwg
机译：领域：药物。物质：将石蜡切片制备，固定并涂上胶体显影剂，该胶体显影剂是将2％的明胶水溶液与1％的甲酸和50％的AgNO 3 水溶液按等比例混合， 30-60分钟，洗涤并对比。该物质在10％中性福尔马林缓冲液（pH 7.4）中固定24小时。该材料在上升的醇中完成，封装在石蜡中以制备切片，该切片在96％乙醇中的2％甲酸中干燥20分钟，然后分两次干燥，每次96％。将切片在0.1％NaOH中制备2分30秒。然后将它们干燥。将一滴100％AgNO 3 滴在制剂上。随后在湿度箱中于60°C的恒温箱中孵育40分钟，持续1分钟40秒，然后用一滴40％甲醛和胶体显影剂进行涂层。在相同温度下，将其在恒温器中孵育20-50秒。将这些部分用4份蒸馏水洗涤。将切片用pH 2.4的醋酸盐缓冲液处理1分钟;将该制剂与0.2％的甲基绿水溶液对比10-15秒，在氯仿中纯化，在正丁醇中分化2-3分钟，在甲苯中加工，并封装在聚苯乙烯中;效果：更高的检测和评估质量核仁组织者。; 1 ex，2 dwg

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