首页> 外国专利> Method for selective separation of target bio-molecules through dielectric make-up either target bio-molecules or the others using nanoparticles by dielectrophoresis

Method for selective separation of target bio-molecules through dielectric make-up either target bio-molecules or the others using nanoparticles by dielectrophoresis

机译:通过介电电泳使用纳米颗粒通过介电构成靶生物分子或其他生物分子选择性分离靶生物分子的方法

摘要

The invention various cells, as for a method for separating biomolecules (bio-molecule) containing viruses, and more particularly to a large number of biomolecules, including the target biomolecule (target bio-molecules) dispersing a fluid sample by a considerably improved over a number of nano-target generated by contact of the particles or molecules remaining biomolecules either one of only doped (doping) inherent characteristics the die electrical property (dielectric property) of the doped biomolecules to ensure that the die electric using a significant difference in characteristics Pori sheath (dielectrophoresis: DEP) only target biomolecule die electroporation is for the target biomolecule separation method using DEP and nanoparticles which can be easily separated by using a. ; By this according to the invention doped with a plurality of nanoparticles to a fluid sample and contacting to one side only of the plurality of nano-of the target biomolecule or the other biomolecule dispersed in the sample fluid particles, the die of the other biomolecules and the target biomolecules since electric characteristics can be induced to have a significant difference, influence by the DEP on the activity of a target biomolecule is while reducing, this not only can improve the separation efficiency of the target biomolecule, the die electrical characteristics of the nanoparticles is very weak, so there is an advantage that little impact when holding a work after the target biomolecules have been separated by using the nano-particles remaining without being doped with the biomolecule, but contact with the fluid sample DEP.
机译:本发明的各种细胞,作为用于分离包含病毒的生物分子(bio-molecules)的方法,更具体地讲,是分离至包括目标生物分子(target bio-moleculars)在内的大量生物分子的方法,通过将液体样品分散在通过接触剩余生物分子的粒子或分子而产生的纳米靶的数量,仅是掺杂(掺杂)固有特性之一,即掺杂生物分子的芯片电性能(介电性能),以确保芯片电特性使用显着差异鞘(介电电泳:DEP)只有目标生物分子电穿孔是针对使用DEP和纳米粒子的目标生物分子分离方法,可以使用a轻松分离。 ;通过根据本发明的此方法,向流体样品中掺杂了多个纳米颗粒,并使仅分散于样品流体颗粒中的靶生物分子或另一种生物分子的多种纳米的一侧,另一种生物分子的模头和与之接触。由于可以诱导目标生物分子具有显着的电特性差异,因此DEP对目标生物分子活性的影响在减小的同时,不仅可以提高目标生物分子的分离效率,还可以提高纳米颗粒的电学特性。纳米粒子非常弱,因此具有的优点是,通过使用未掺杂生物分子但与流体样品DEP接触而保留的纳米粒子,在分离目标生物分子后保持工作时几乎没有影响。

著录项

  • 公开/公告号KR101138868B1

    专利类型

  • 公开/公告日2012-05-14

    原文格式PDF

  • 申请/专利权人

    申请/专利号KR20050077366

  • 发明设计人 조윤경;정성영;이규상;

    申请日2005-08-23

  • 分类号C12N13/00;C12N9/00;

  • 国家 KR

  • 入库时间 2022-08-21 17:08:13

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