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METHOD FOR DETECTING AN ENDOPLASMIC RETICULUM(ER) STRESS INDUCER USING HUMAN EPITHELIAL CELL LINE

机译:人上皮细胞系检测内质网应激诱导剂的方法

摘要

PURPOSE: A method for detecting an ER stress inducer using SEAP expression epithelial cell line is provided to accurately induce ER stress.;CONSTITUTION: An alkali phosphatase expression vector of a secretory type contains SV40 promoter, hygromycin gene, and alkali phosphatase gene. A human epithelial cell line which always expresses SEAP(secreted alkaline phosphatase) contains the expression vector. The epithelial cell line is an intestine epithelial cell. A composition for detecting the ER stress inducer contains the epithelial cell line. The ER stress inducer is fungal toxin, 8-keto trichothecen. A method for detecting the ER stress inducer comprises: a step of adding a sample to the composition for reaction; and a step of observing secretion change of the SEAP.;COPYRIGHT KIPO 2012
机译:目的:提供一种利用SEAP表达上皮细胞系检测ER应激诱导剂的方法,以准确诱导ER应激。;组成:分泌型碱性磷酸酶表达载体包含SV40启动子,潮霉素基因和碱性磷酸酶基因。始终表达SEAP(分泌的碱性磷酸酶)的人上皮细胞系包含表达载体。上皮细胞系是肠上皮细胞。用于检测ER应激诱导剂的组合物包含上皮细胞系。内质网应激诱导物是真菌毒素8-酮三色氨酸。一种用于检测ER应激诱导剂的方法,包括:将样品加入到组合物中进行反应的步骤;以及观察SEAP分泌变化的步骤。; COPYRIGHT KIPO 2012

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