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METHOD FOR DETECTING AN ENDOPLASMIC RETICULUM(ER) STRESS INDUCER USING HUMAN EPITHELIAL CELL LINE
METHOD FOR DETECTING AN ENDOPLASMIC RETICULUM(ER) STRESS INDUCER USING HUMAN EPITHELIAL CELL LINE
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机译:人上皮细胞系检测内质网应激诱导剂的方法
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摘要
PURPOSE: A method for detecting an ER stress inducer using SEAP expression epithelial cell line is provided to accurately induce ER stress.;CONSTITUTION: An alkali phosphatase expression vector of a secretory type contains SV40 promoter, hygromycin gene, and alkali phosphatase gene. A human epithelial cell line which always expresses SEAP(secreted alkaline phosphatase) contains the expression vector. The epithelial cell line is an intestine epithelial cell. A composition for detecting the ER stress inducer contains the epithelial cell line. The ER stress inducer is fungal toxin, 8-keto trichothecen. A method for detecting the ER stress inducer comprises: a step of adding a sample to the composition for reaction; and a step of observing secretion change of the SEAP.;COPYRIGHT KIPO 2012
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