首页> 外国专利> SCREENING METHOD FOR THE IDENTIFICATION OF TNF-PATHWAY MODULATORS USING TRADD/ REPORTER FUSION PROTEIN.

SCREENING METHOD FOR THE IDENTIFICATION OF TNF-PATHWAY MODULATORS USING TRADD/ REPORTER FUSION PROTEIN.

机译:使用贸易/报告融合蛋白鉴定TNF途径调节剂的筛选方法。

摘要

The present invention relates inter alia to methods for identifying modulators of tumour necrosis factor (TNF) signalling. In one aspect, the method involves identifying an agent that modulates a signalling pathway mediated by TNF, comprising the steps of providing a host cell comprising TNF receptor 1 (TNFR1)-associated death domain (TRADD) linked to a reporter molecule, and determining at least one cellular characteristic detectable by the reporter molecule in the host cell in the presence of TNF and in the presence and absence of a candidate modulator. The invention is suitable for high-throughput screening (HTS) and high-content screening (HCS), or a combination of HTS and HCS.
机译:本发明尤其涉及用于鉴定肿瘤坏死因子(TNF)信号传导的调节剂的方法。一方面,该方法涉及鉴定调节由TNF介导的信号传导途径的试剂,其包括以下步骤:提供包含与报道分子连接的TNF受体1(TNFR1)相关的死亡域(TRADD)的宿主细胞,并确定在TNF存在下和候选调节剂存在与否下,宿主细胞中的报告分子可检测到的至少一种细胞特征。本发明适用于高通量筛选(HTS)和高含量筛选(HCS),或HTS和HCS的组合。

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