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INDUCTION OF MUCOSAL IMMUNE RESPONSES BY MUCOSAL DELIVERY PENTABODY COMPLEX (MDPC)

机译:粘膜递送五种复合物(MDPC)对粘膜免疫反应的诱导

摘要

The subject invention provides, for example, a novel approach to specifically induce intranasal and/or oral mucosal as well as humoral antibody response by administrating a mucosal delivery pentabody complex (MDPC). The MDPC is a complex formed by mixing a target antigen and a mucosal delivery pentabody (MDP) that has a strong affinity to the target antigen. The MDP is a fusion protein of a single domain antibody (sdAb; which binds to the target antigen specifically) to a pentamerization domain (which can include the B-subunit of an AB5 toxin family, including the B subunit of cholera toxin (CT) or heat-labile toxin (LT)). The pentamerization domain can self-assemble into a pentamer, through which a pentameric single domain antibody, or a pentabody, is formed.
机译:本发明提供例如新颖的方法,该方法通过施用粘膜递送五聚体复合物(MDPC)来特异性诱导鼻内和/或口腔粘膜以及体液抗体应答。 MDPC是通过将靶抗原和对靶抗原具有强亲和力的粘膜递送五聚体(MDP)混合而形成的复合物。 MDP是单结构域抗体(sdAb;与目标抗原特异性结合)与五聚化结构域(可包括AB5毒素家族的B亚基,包括霍乱毒素(CT)的B亚基)的融合蛋白。或不耐热毒素(LT))。五聚化结构域可以自组装成五聚体,通过该五聚体形成五聚体单结构域抗体或五聚体。

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