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METHODS AND APPARATUS RELATING TO INTEGRATED NIR FLUORESCENCE AND REFLECTANCE IMAGING AND SPECTROSCOPY WITH POLARIZATION FOR IMPROVING TISSUE DIAGNOSIS AND CHARACTERIZATION
METHODS AND APPARATUS RELATING TO INTEGRATED NIR FLUORESCENCE AND REFLECTANCE IMAGING AND SPECTROSCOPY WITH POLARIZATION FOR IMPROVING TISSUE DIAGNOSIS AND CHARACTERIZATION
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机译：结合近红外荧光和反射成像及光谱的极化的方法和装置，用于改善组织的诊断和鉴定
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Methods and apparatus relating to integrated NIR fluorescence and reflectance imaging and spectroscopy with polarization for improving tissue diagnosis and characterizationAbstract We report on the development of the near-infrared (NIR) autofluorescence (AF) spectroscopy technique for in vivo identification of neoplastic polyps in the colon at colonoscopy. In vivo NIR AF spectra from colonic tissue can be acquired within 1 second using the 785 nm laser excitation power of 1.0 W/cm2. Significant differences in in vivo NIR AF spectra are observed among normal (n=116), hyperplastic polyp (n=48) and adenomatous polyp (n=34) colonic tissue from 96 patients. The multivariate analysis [i.e., principal components analysis (PCA) and linear discriminate analysis (LDA)], together with the leave-one tissue site-out, cross validation on in vivo NIR AF spectra yields a predictive accuracies of 90%, 82.6% and 90.2%, respectively, for differentiation among normal, hyperplastic and adenomatous polyps in the colon. This work demonstrates for the first time that NIR AF spectroscopy associated with PCA-LDA diagnostic algorithms has potential for the noninvasive, in vivo diagnosis of neoplastic polyps in the colon during clinical colonoscopy screening. In the mean time, we have further evaluated the diagnostic feasibility of the integrated near-infrared (NIR) autofluorescence (AF) and NIR diffuse reflectance (DR) imaging technique developed for colonic cancer detection. A total of 48 paired colonic tissue specimens (normal vs. cancer) were measured using the integrated NIR DR (850-1100 nm) and NIR AF imaging at the 785 nm laser excitation. The results showed that NIR AF intensities of cancer tissues are significantly lower than those of normal tissues (p0.001, paired 2-sided Student's t-test, n=48). NIR AF imaging under polarization conditions gives a higher diagnostic accuracy (of -92-94%) compared to non-polarized NIR AF imaging or NIR DR imaging. Further, the ratio imaging of NIR DR to NIR AF with polarization provides the best diagnostic accuracy (of -96%) among the NIR AF and NIR DR imaging techniques. This work suggests that the integrated NIR AF spectroscopy and NIR AF/DR imaging under polarization condition has the potential to greatly improve the early diagnosis and detection of malignant lesions in the colon.Figure 1.
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