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Recombinant plant comprising the insertion of a native genetic element without the insertion of border sequences or selectable markers

机译:重组植物,包括插入天然遗传元件而没有插入边界序列或选择标记

摘要

Disclosed is a transgenic plant: (a) which has been derived from a non-transgenic plant by stably transforming the non-transgenic plant with a desired polynucleotide consisting essentially of one or more native genetic elements isolated from: (i) the non-transgenic plant; (ii) a plant of the same species as the non-transgenic plant; or (iii) a plant that is sexually interfertile with the non-transgenic plant; and wherein the desired polynucleotide does not comprise a nucleotide sequence that is identical in nucleotide sequence to an Agrobacterium derived T-DNA border sequence; and (b) in which the desired polynucleotide modifies the expression of a trait in the transgenic plant compared to the expression of the trait in the non-transgenic plant.
机译:公开了一种转基因植物:(a)通过用所需的多核苷酸稳定转化非转基因植物而衍生自非转基因植物,所述所需的多核苷酸基本上由一种或多种天然遗传元件组成,所述所需多核苷酸选自:(i)非转基因厂; (ii)与非转基因植物同种的植物; (iii)与非转基因植物发生性育的植物;或其中所需的多核苷酸不包含与农杆菌衍生的T-DNA边界序列在核苷酸序列上相同的核苷酸序列; (b)与非转基因植物中性状的表达相比,所需的多核苷酸修饰了转基因植物中性状的表达。

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