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The method of quantification of vitamin C in Strawberry

机译:草莓中维生素C的定量方法

摘要

The method of quantification of vitamin C in strawberry, this invention has developed a methodology for quantifying vitamin C by high performance liquid chromatography (HPLC) using pre - column and reverse phase column (250 x 4,6 mm; 5pmid). 100% of a buffer pH 6,67 containing sodium acetate 0.04 mol.l1, EDTA acid 0.05 mol.l1, tetrabutylammonium phosphate 0.5 mol.L1 (1: 1: 1) as mobile phase, mode isocru00e1tico, flow 0,6 ml mm 1 and a UV detector at a wavelength of 254 nm.The developed methodology has provided good resolution between the peaks, the accuracy of the method was 95 to 100% used as the mobile phase aqueous solutions highly diluted with pH 6,67, reagents of low risk, at ambient temperature (25 198 C) and also optimized the analysis time.
机译:草莓中维生素C的定量方法,本发明开发了一种通过使用前柱和反相柱(250 x 4,6 mm; 5pmid)的高效液相色谱(HPLC)定量维生素C的方法。 100%的pH 6,67缓冲液,其中包含乙酸钠0.04 mol.l,EDTA酸0.05 mol.l,磷酸四丁铵0.5 mol.L1(1:1:1)作为流动相,等压模式,流量0.6 ml mm 1和254 nm波长的紫外检测器。开发的方法在峰之间提供了良好的分离度,该方法的准确度为95至100%,用作pH 6,67高度稀释的流动相水溶液,低风险的试剂,在环境温度(25 <198> C)下,还优化了分析时间。

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