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首页> 外国专利> Isolated nucleic acid molecules from transgenic papaya line 16-0-1 resistant to papaya ringspot virus and use thereof

Isolated nucleic acid molecules from transgenic papaya line 16-0-1 resistant to papaya ringspot virus and use thereof

机译：抗木瓜环斑病毒的转基因木瓜株系16-0-1的分离核酸分子及其用途

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摘要

Provided is an isolated nucleic acid molecule having a right border flanking region, a left border flanking region and a transgene sequence between the right border flanking region and the left border flanking region, wherein the right border flanking region having at least 90% homology with the sequence set forth in SEQ ID NO: 29; the left border flanking region having at least 90% homology with the sequence set forth in SEQ ID NO: 31; and the transgene sequence having a papaya ringspot virus coat protein gene and a promoter operably linked to the papaya ringspot virus coat protein gene. Primers, probes and kit derived from the isolated nucleic acid molecule are proved to be useful for identifying the transgenic papaya line 16-0-1 in a specific, reproducible, sensitive and reliable way.
机译：提供了一种分离的核酸分子，其具有右边界侧翼区，左边界侧翼区和在右边界侧翼区与左边界侧翼区之间的转基因序列，其中右边界侧翼区与DNA具有至少90％的同源性。 SEQ ID NO：29所示的序列;左边界侧翼区与SEQ ID NO：31所示的序列具有至少90％的同源性;该转基因序列具有木瓜环斑病毒外壳蛋白基因和与木瓜环斑病毒外壳蛋白基因可操作地连接的启动子。事实证明，衍生自分离的核酸分子的引物，探针和试剂盒可用于以特定，可再现，敏感和可靠的方式鉴定转基因木瓜品系16-0-1。

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