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Myelination of congenitally dysmyelinated forebrains using oligodendrocyte progenitor cells

机译:使用少突胶质细胞祖细胞对先天性髓鞘异常的前脑进行髓鞘形成

摘要

One form of the present invention is directed to a method of remyelinating demyelinated axons by treating the demyelinated axons with oligodendrocyte progenitor cells under conditions which permit remyelination of the axons. Another aspect of the present invention relates to a method of treating a subject having a condition mediated by a loss of myelin or a loss of oligodendrocytes by administering to the subject oligodendrocyte progenitor cells under conditions effective to treat the condition mediated by a loss of myelin or a loss of oligodendrocytes. A further aspect of the present invention relates to an in vitro method of identifying and separating oligodendrocyte progenitor cells from a mixed population containing other mammalian brain or spinal cord cell types. This further aspect of the present invention involves removing neurons and neuronal progenitor cells from the mixed population to produce a treated mixed population. Oligodendrocyte progenitor cells are then separated from the treated mixed population to form an enriched population of oligodendrocyte progenitor cells.
机译:本发明的一种形式涉及通过在允许轴突再髓鞘化的条件下用少突胶质细胞祖细胞处理脱髓鞘的轴突来使脱髓鞘的轴突再髓鞘化的方法。本发明的另一方面涉及通过在有效治疗由髓磷脂或β-脱氢酶介导的病症的条件下向受试者施用少突胶质细胞祖细胞来治疗患有由髓磷脂的丧失或少突胶质细胞的丧失介导的病症的受试者的方法。少突胶质细胞丢失。本发明的另一方面涉及从包含其他哺乳动物脑或脊髓细胞类型的混合群体中鉴定和分离少突胶质祖细胞的体外方法。本发明的另一方面涉及从混合群体中除去神经元和神经元祖细胞以产生经处理的混合群体。然后从处理过的混合群体中分离少突胶质祖细胞,以形成少突胶质祖细胞的富集群体。

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