• 主题
高级搜索  >
历史搜索 清空历史
首页> 外国专利> Modifying substrate specificity of polyol oxidase, comprises providing the polyol oxidase comprising specified amino acid sequences and introducing specified amino acid substitutions in the polyol oxidase

Modifying substrate specificity of polyol oxidase, comprises providing the polyol oxidase comprising specified amino acid sequences and introducing specified amino acid substitutions in the polyol oxidase

机译：改变多元醇氧化酶的底物特异性，包括提供包含特定氨基酸序列的多元醇氧化酶并在多元醇氧化酶中引入特定氨基酸取代

页面导航
  • 摘要
  • 著录项
  • 相似文献

摘要

Method for modifying substrate specificity of polyol oxidase, comprises (a) providing the polyol oxidase comprising an amino acid sequence that is at least 85% identical to the amino acid sequence of SEQ ID NO: 1 (where sequences are not defined), and (b) introducing one or more amino acid substitutions in the count according to SEQ ID NO: 1 in the polyol oxidase. Method for modifying substrate specificity of polyol oxidase, comprises (a) providing the polyol oxidase comprising an amino acid sequence that is at least 85% identical to the amino acid sequence of SEQ ID NO: 1 (where sequences are not defined), and (b) introducing one or more amino acid substitutions of Ala-64-Val, Val-125-Met, Val-133-Met, Gly-236-Asp, Ala-244-Thr, Gly-399-Trp and/or Gly-399-Arg in the count according to SEQ ID NO: 1 in the polyol oxidase, where the modified substrate specificity of polyol oxidase after the step (b) is determined against the polyol oxidase after the step (a) with a polyalcohol as substrate for the polyol oxidase. Independent claims are included for: (1) the polyol oxidase exhibiting modified substrate specificity compared to the polyol oxidase comprising amino acid sequences of SEQ ID NO: 1, with a polyalcohol as a substrate for polyol oxidase; (2) a nucleic acid encoding the polyol oxidase; (3) a non-human host cell comprising the nucleic acid or a vector or the polyol oxidase; (4) producing the polyol oxidase, comprising culturing the host cell, and isolating polyol oxidase from the culture medium or from the host cell; (5) an agent, preferably a washing or cleaning agent, bleaching agent, disinfectant or cosmetic agent, comprising the polyol oxidase and a substrate for the polyol oxidase at a concentration of 2 mu g to 20 mg (preferably 50 mu g to 10 mg) per g of the agent, where the polyol oxidase in the agent is wrapped with a substance impermeable to the polyol oxidase at room temperature or in the absence of water; and (6) a method for enzymatic production of hydrogen peroxide, preferably in a washing liquor, bleaching composition or disinfectant composition, comprising applying the agent in at least one of the process steps, or catalytically activating the polyol oxidase in the presence of a substrate for the polyol oxidase, where 40 mu g to 4 g (preferably 200 mu g to 1 g) of polyol oxidase is used per application.
机译：修饰多元醇氧化酶的底物特异性的方法，包括（a）提供一种多元醇氧化酶，该多元醇氧化酶的氨基酸序列与SEQ ID NO：1的氨基酸序列具有至少85％的同一性（未定义序列），和（ b）在多元醇氧化酶的根据SEQ ID NO：1的计数中引入一个或多个氨基酸取代。修饰多元醇氧化酶的底物特异性的方法，包括（a）提供一种多元醇氧化酶，该多元醇氧化酶的氨基酸序列与SEQ ID NO：1的氨基酸序列具有至少85％的同一性（未定义序列），和（ b）引入一个或多个氨基酸取代的Ala-64-Val，Val-125-Met，Val-133-Met，Gly-236-Asp，Ala-244-Thr，Gly-399-Trp和/或Gly-多元醇氧化酶中根据SEQ ID NO：1的计数为399-Arg，其中相对于步骤（a）之后的多元醇氧化酶，以多元醇为底物，测定了步骤（b）之后多元醇氧化酶的修饰底物特异性。多元醇氧化酶。包括以下独立权利要求：（1）与包含SEQ ID NO：1的氨基酸序列的多元醇氧化酶相比，多元醇氧化酶具有改进的底物特异性，其中多元醇作为多元醇氧化酶的底物; （2）编码多元醇氧化酶的核酸。 （3）包含所述核酸或载体或所述多元醇氧化酶的非人类宿主细胞。 （4）生产多元醇氧化酶，其包括培养宿主细胞，并从培养基或宿主细胞中分离多元醇氧化酶; （5）包含多元醇氧化酶和多元醇氧化酶底物的试剂，优选为洗涤或清洁剂，漂白剂，消毒剂或美容剂，其浓度为2μg至20mg（优选为50μg至10mg） ）每克试剂，其中试剂中的多元醇氧化酶在室温下或在没有水的情况下被不透多元醇氧化酶的物质包裹着; （6）优选在洗涤液，漂白组合物或消毒剂组合物中酶促生产过氧化氢的方法，其包括在至少一个工艺步骤中施用所述试剂，或在底物存在下催化活化多元醇氧化酶。对于多元醇氧化酶，其中每次应用使用40μg至4g（优选200μg至1g）的多元醇氧化酶。

著录项

相似文献

  • 专利
  • 外文文献
  • 中文文献
获取专利

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号