首页> 外国专利> Water treatment plant comprises biosensor that has genetically modified yeast comprising e.g. first expression cassette for expression of retinoid X receptor gene, and second expression cassette for expression of pregnane X receptor gene

Water treatment plant comprises biosensor that has genetically modified yeast comprising e.g. first expression cassette for expression of retinoid X receptor gene, and second expression cassette for expression of pregnane X receptor gene

机译:水处理厂包括生物传感器,该生物传感器具有转基因酵母,所述酵母包括例如酵母。第一个表达盒用于表达类视黄醇X受体基因,第二个表达盒用于表达孕烷X受体基因

摘要

Water treatment plant with an oxidation device with an inlet for water to be treated and an outlet for treated water, and a dosage device for the addition of oxidant, comprises a biosensor that has a genetically modified yeast comprising a first expression cassette for constitutive expression of retinoid X receptor (RXR)-gene, a second expression cassette for constitutive expression of a pregnane X receptor (PXR)-gene and a third expression cassette with a reporter gene under the control of a promoter which has at least one response element. Water treatment plant with an oxidation device with an inlet for water to be treated and an outlet for treated water, and a dosage device for the addition of oxidant, comprises a biosensor that has a genetically modified yeast comprising a first expression cassette for constitutive expression of retinoid X receptor (RXR)-gene, a second expression cassette for constitutive expression of a pregnane X receptor (PXR) gene and a third expression cassette with a reporter gene under the control of a promoter which has at least one response element, which is specifically in the presence of a drug for the dimer from the translation product of the RXR-gene and the PXR-gene, where the biosensor is equipped with a detector for detecting the translation product of the reporter gene or the conversion of a precursor compound by a product of the reporter gene, to generate a measurement signal based on the detection, and the dosage device for oxidant is coupled with the biosensor and is controlled by it. Independent claims are included for: (1) controlling a device for water treatment, comprising an oxidation device, with addition of oxidant in the oxidation device, where the addition of the oxidant is controlled depending on a measured signal, which is obtained by detection of a product of a reporter gene expressed by a yeast, which is arranged in a third expression cassette under the control of a promoter that exhibits at least one response element, which contains a specific sequence for the addition of a dimer; and (2) the genetically modified yeast for use in a device.
机译:具有氧化装置的水处理厂,其具有用于处理水的入口和用于处理水的出口以及用于添加氧化剂的剂量装置,该生物处理装置具有生物传感器,该生物传感器具有转基因酵母,该酵母包含用于组成型表达的第一表达盒。类视黄醇X受体(RXR)基因,用于组成型表达孕烷X受体(PXR)基因的第二表达盒和具有带有至少一个响应元件的启动子控制的报道基因的第三表达盒。具有氧化装置的水处理厂,其具有用于处理水的入口和用于处理水的出口以及用于添加氧化剂的剂量装置,该生物处理装置具有生物传感器,该生物传感器具有转基因酵母,该酵母包含用于组成型表达的第一表达盒。类视黄醇X受体(RXR)基因,用于组成型表达孕烷X受体(PXR)基因的第二个表达盒和带有报告基因的第三种表达盒,在带有至少一个应答元件的启动子的控制下,具体地,在存在来自RXR基因和PXR基因的翻译产物的二聚体的药物的情况下,其中生物传感器配备有检测器,用于检测报告基因的翻译产物或前体化合物的转化。报告基因的产物,根据检测结果产生测量信号,氧化剂剂量装置与生物传感器耦合,并由t。包括以下独立权利要求:(1)控制用于水处理的设备,该设备包括氧化设备,并且在氧化设备中添加氧化剂,其中氧化剂的添加取决于通过检测到的信号获得的测量信号。由酵母表达的报道基因的产物,其在显示至少一个应答元件的启动子的控制下排列在第三表达盒中,所述启动子具有至少一个用于添加二聚体的特定序列; (2)用于装置的转基因酵母。

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