首页> 外国专利> STRAIN Streptoalloteichus cremeus subsp tobramycini ALL-RUSSIAN COLLECTION OF INDUSTRIAL MICROORGANISMS Ac-1083 - PRODUCENT OF TOBRAMYCIN AND METHOD OF OBTAINING IT

STRAIN Streptoalloteichus cremeus subsp tobramycini ALL-RUSSIAN COLLECTION OF INDUSTRIAL MICROORGANISMS Ac-1083 - PRODUCENT OF TOBRAMYCIN AND METHOD OF OBTAINING IT

机译：菌株Streptoalloteichus cremeus亚种tobramycini工业微生物Ac-1083的全俄文集-Tobramycin的生产及其获得方法

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FIELD: medicine.;SUBSTANCE: strain Streptoalloteichus cremeus subsp.tobramycini 8150 All-Russian collection of industrial microorganisms Ac-1083 is obtained as a result of induced mutagenesis with further selection of variants on selective media, containing streptomycin, rifamycin and tobramycin. Method of obtaining tobramycin from culture liquid of novel strain with application of column chromatography on carboxyl cationites with step-by-step elusion with ammonia solutions, hydrolysis at 100°C in (0.1-0.3) H solution of caustic soda, repeated chromatographic purification, concentration in vacuum, acidation with solution of sulfuric acid to pH 4.5 and sedimentation with metghanol in form of sulfate provides 30% output of target product from tombamycin content in culture liquid.;EFFECT: invention makes it possible to increase efficiency of antibiotic obtaining.;2 cl, 1 tbl, 1 ex

机译：领域：医学;物质：菌株Streptoalloteichus cremeus亚种tobramycini 8150由于诱变并在包括链霉素，利福霉素和妥布霉素在内的选择性培养基上进一步选择了变异体，因此获得了全俄工业微生物Ac-1083的收集。从新菌株的培养液中获得妥布霉素的方法是，在羧基阳离子上应用柱色谱，逐步用氨水洗脱，在苛性钠的（0.1-0.3）H溶液中于100°C水解，重复色谱纯化，真空浓缩，用硫酸溶液酸化至pH 4.5，并用硫酸甲醇形式的甲醇沉淀，从培养液中的汤姆霉素含量中得到目标产物的30％。效果：本发明可以提高抗生素的获得效率。 2 cl，1 tbl，1前

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公开/公告号RU2433169C1

专利类型
公开/公告日2011-11-10

原文格式PDF
申请/专利权人 UCHREZHDENIE ROSSIJSKOJ AKADEMII MEDITSINSKIKH NAUK NAUCHNO-ISSLEDOVATELSKIJ INSTITUT PO IZYSKANIJU NOVYKH ANTIBIOTIKOV IM. G.F. GAUZE ROSSIJSKOJ AKADEMII MEDITSINSKIKH NAUK (NIINA RAMN);
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申请/专利号RU20100129776
发明设计人 LAVROVA-BALASHOVA MAJJA FEDOROVNA;PONOMARENKO VALERIJ IVANOVICH;FILICHEVA VALENTINA ANDREEVNA;KHARITONOVA LIDIJA ALEKSANDROVNA;POGOZHEVA VALERIJA VLADIMIROVNA;LAPCHINSKAJA OLDA ANASTASEVNA;PREOBRAZHENSKAJA MARIJA NIKOLAEVNA;GLADKIKH ELENA GEORGIEVNA;MAKAROVA MARINA OLEGOVNA;FEDOROVA GALINA BORISOVNA;ORLOVA GALINA IVANOVNA;KATRUKHA GENRIKH STEPANOVICH;
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申请日2010-07-20
分类号C12N1/14;C12P1/06;C12R1/01;
国家 RU
入库时间 2022-08-21 17:48:13

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