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首页> 外国专利> OLIGONUCLEOTIDE PRIMERS, METHOD AND TEST SYSTEM FOR IDENTIFYING GENOME OF RABBIT VIRAL HEMORRHAGIC DISEASE BY REVERSE TRANSCRIPTION - POLYMERASE CHAIN REACTION

OLIGONUCLEOTIDE PRIMERS, METHOD AND TEST SYSTEM FOR IDENTIFYING GENOME OF RABBIT VIRAL HEMORRHAGIC DISEASE BY REVERSE TRANSCRIPTION - POLYMERASE CHAIN REACTION

机译：逆转录-聚合酶链反应鉴定兔病毒性血液病基因组的寡核苷酸起点，方法和试验系统

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FIELD: medicine.;SUBSTANCE: invention refers to synthetic oligonucleotide primers, complementary to high conservative VP60 gene region of a genome of rabbit viral hemorrhagic disease virus, to a method for identifying rabbit viral hemorrhagic disease virus and to a test system for identifying RNA of rabbit viral hemorrhagic disease virus. The offered invention can be used in veterinary virology. The method for identifying rabbit viral hemorrhagic disease virus involves sample preparation, RNA recovery from the biological material. It is followed with conducting a polymerase chain reaction with using primers 5'-caa cgt get cca gtt ttg gta cg-3', 5'-att ctg tct ggt tgg ggc gtg t-3'. Further, viral RNA is amplified. Then, the reaction is assessed by agarose gel electrophoresis, with a reaction result considered as positive if the PCR product corresponds to the size of 398 base pairs.;EFFECT: invention allows higher sensitivity of the method, as well as reduced time of diagnostic manipulations with organ and blood samples of the infected animals.;3 cl, 4 tbl, 4 ex
机译：技术领域本发明涉及合成的寡核苷酸引物，其与兔病毒性出血病病毒基因组的高保守VP60基因区互补，鉴定兔病毒性出血病病毒的方法和鉴定该病毒的RNA的测试系统。兔病毒性出血性疾病病毒。所提供的发明可以用于兽医病毒学。鉴定兔病毒性出血性疾病病毒的方法涉及样品制备，从生物材料中回收RNA。随后使用引物5'-caa cgt get cca gtt ttg gta cg-3'，5'-att ctg tct ggt tgg ggc gtg t-3'进行聚合酶链反应。此外，病毒RNA被扩增。然后，通过琼脂糖凝胶电泳评估反应，如果PCR产物对应于398个碱基对的大小，则反应结果为阳性。效果：本发明允许该方法具有更高的灵敏度，并减少了诊断操作的时间以及被感染动物的器官和血液样本。; 3 cl，4 tbl，4 ex

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