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Embryonic Callus Induction and Plant Regeneration Methods from Mature Seed of Miscanthus sinensis
Embryonic Callus Induction and Plant Regeneration Methods from Mature Seed of Miscanthus sinensis
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机译：芒草成熟种子的胚性愈伤组织诱导和植株再生方法
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摘要
PURPOSE: A method for inducing embryonic callus and redifferentiating plant from mature seed of Miscanthus sinensis is provided to improve induction efficiency and differentiation efficiency. CONSTITUTION: An embryonic callus is induced by culturing mature seeds of Miscanthus sinensis in an MS medium containing 25-35g/L of sucrose, 700-800 mg/L of MgCl_26H_2O, 1-3 g/L of gellite, 2-4mg/L of 2,4-D. A medium for inducing embryonic callus contains N6 medium instead of MS medium. An embryonic callus is induced at 22-26C and 40~60 uE/m^2s for 3 to 5 weeks. A plant is redifferentiated by culturing the embryonic callus in an MS medium containing 25-35 g/L of sucrose, 700-800 mg/mL of MgCl_26H_2O, 1-3 g/L of gellite, 1-3 mg/L of 2,4-D and 1.5-2.5 mg/L of BA. The redifferentiation is performed at 22-26C during daytime for 14-16 hours and at night for 8-10 hours.
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机译：目的：提供一种从中国芒草成熟种子中诱导愈伤组织和再分化植物的方法，以提高其诱导效率和分化效率。组成：胚愈伤组织是通过在含25-35g / L蔗糖，700-800 mg / L MgCl_26H_2O，1-3 g / L胶体，2-4mg / L的MS培养基中培养芒草的成熟种子而诱导的。 2,4-D。诱导胚性愈伤组织的培养基包含N6培养基而不是MS培养基。在22-26°C和40〜60 uE / m ^ 2s的条件下诱导胚愈伤组织3至5周。通过在含有25-35 g / L蔗糖，700-800 mg / mL MgCl_26H_2O，1-3 g / L胶凝剂，1-3 mg / L的2的MS培养基中培养胚愈伤组织来重新分化植物。 4-D和1.5-2.5 mg / L BA。再分化在白天在22-26°C下进行14-16小时，在夜间进行8-10小时。
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