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Method for detecting methylation of gastric cancer specific methylation marker gene for gastric cancer diagnosis

机译:检测用于胃癌诊断的胃癌特异性甲基化标记基因甲基化的方法

摘要

PURPOSE: A method for detecting methylation of gastric cancer-specific methylated marker gene is provided to quickly and accurately diagnose gastric cancer. CONSTITUTION: A method for detecting methylation is PCR, methylateion-specific PCR, real time methylation-specific PCR, PCR using methylation DNA-specific binding protein, quantitative PCR, pyrosequencing or bisulfite sequencing. A promoter is a sequence of sequence number 1. A kit for diagnosing gastric cancer or progression of the cancer comprises: primer pair for amplifying a gastric cancer specific-methylation marker gene COL24A1(NM_152890, collagen type 24, alpha 1) promoter CpG island; and methylation-sensitive restriction endonuclease. The endonuclease is SmaI, HpaII, BssHII, BstUI, NotI, SacII or EagI.
机译:目的:提供一种检测胃癌特异性甲基化标记基因甲基化的方法,以快速,准确地诊断胃癌。组成:检测甲基化的方法是PCR,甲基化特异性PCR,实时甲基化特异性PCR,使用甲基化DNA特异性结合蛋白的PCR,定量PCR,焦磷酸测序或亚硫酸氢盐测序。启动子是序列号为1的序列。和甲基化敏感的限制性核酸内切酶。核酸内切酶是SmaI,HpaII,BssHII,BstUI,NotI,SacII或EagI。

著录项

  • 公开/公告号KR101009062B1

    专利类型

  • 公开/公告日2011-01-17

    原文格式PDF

  • 申请/专利权人

    申请/专利号KR20100093976

  • 申请日2010-09-28

  • 分类号C12Q1/68;C12N15/12;G06F19/20;

  • 国家 KR

  • 入库时间 2022-08-21 17:50:40

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