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DEVELOPMENT OF KOREAN NATIVE PIGS SPECIFIC MOLECULAR MARKER AND METHOD FOR DETECTING KOREAN NATIVE PIGS USING THE MARKER
DEVELOPMENT OF KOREAN NATIVE PIGS SPECIFIC MOLECULAR MARKER AND METHOD FOR DETECTING KOREAN NATIVE PIGS USING THE MARKER
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机译:韩国本地猪特定分子标记的开发以及使用该标记检测韩国本地猪的方法
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摘要
PURPOSE: a kind of method that exploitation uses it for Korean tradition pig molecule mark and determining Korea's tradition pig. ;CONSTITUTION: it is a kind of distinguish Korean tradition pig variety and improve pig variety include: step KIT genes amplification Korean traditional pig variety and improve pig variety (local varieties and Large White) use primer Serial No. 5 and 6; One step, carrying out PCR-RFLP (restriction fragment length polymorphism) includes the processing of ACC II restriction endonuclease. The primer has the base sequence of sequence number 5, GTAAGGCCCAGATGTTCTTCTT with a forward primer; There is the base sequence of sequence number 6, CAACCGGACCTACTGAAATGAC with reverse primer. ;The 2011 of copyright KIPO submissions
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