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METHOD FOR TREATING ALLOGENIC SKIN AND CRYO-PRESERVED ALLOGENIC SKIN THEREBY
METHOD FOR TREATING ALLOGENIC SKIN AND CRYO-PRESERVED ALLOGENIC SKIN THEREBY
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机译:治疗同种异体皮肤并由此冷冻保存的同种异体皮肤的方法
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摘要
PURPOSE: A method for treating allogenic skin and cryo-preserved allogenic skin are provided to enhance survival rate of cells and to help tissue generation in a short time.;CONSTITUTION: A method for treating allogenic skin comprises: a step of mixing DMSO, animal cell culture medium and FBS; a step of dissolving sucrose in the mixture solution to obtain cryoprotectant; a step of penetrating the cryoprotectant onto the skin; and a step of freezing the skin in a controlled rate freezer. The final concentration of sucrose is 30 weight%. The animal cell culture medium is MEM, DMEM, RPMI 1640 or IMDM.;COPYRIGHT KIPO 2011
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