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Development of molecular markers linked to the genic male sterility in Paprika colored sweet pepper and their use for developing new GMS inbred lines
Development of molecular markers linked to the genic male sterility in Paprika colored sweet pepper and their use for developing new GMS inbred lines
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机译：与辣椒粉有色雄性不育相关的分子标记的开发及其在开发新的GMS自交系中的应用
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摘要
invention paprika (sweet pepper coloring) in the six kinds of heterosis in hybrid seed production and for use by one and that genetic male sterility (GMS) to develop molecular markers associated with the development in the short term a new system and paprika varieties selected by early to determine the desired male sterility genotype in the development process of new strains and varieties and using them relates to the strategy. ; The method has been used in one hybrid seed production of three paprika pepper significantly (colored sweet peppers) the absence of one parent hybrid seeds recovery and instability in the production of male sterility Due to the cytoplasmic-genetic male sterility because (CGMS) do not use the F using genetic male sterility (GMS), or by mating jeung 1 and produce varieties of seeds. All the genetic male sterility gene that are known or used so far are genetically recessive. Thus, new varieties and genetic male sterility system (GMS) in the breeding process when we want to introduce the system, you should use a recessive gene backcrossing breeding. In this breeding process, we can easily distinguish with the naked eye fertile state and infertility weeks that make up the separate groups. However, homozygous in the traditional way to what we want by selecting from among the heterozygous fertile entities to the new system it should be immobilized through the ongoing child or backcrossing and breeding hyeongmae (homozygote, MsMs ) and heterozygotes (heterozygote, Msms ) can not be classified as a contemporary expression in the later starters should always desired object through the heterozygous black. Thus, six kinds of light are needed to develop new varieties is of course much longer will it require a large workforce and packaging. ; In order to solve these problems develop molecular markers associated with male sterility and use them breeding process During each object to determine the genotype between generations must develop a technology capable of selecting a desired genotype in his time. Therefore, in the present invention, which is imported from Europe paprika (sweet pepper coloring) F 1 to develop molecular genetic markers associated with male infertility being used to develop new varieties and effectiveness by using this system and breed It includes those related to the method and strategy to maximize. In other words, the present invention is commercially paprika F 1 Self modified varieties (selfing) made by F 2 in separate groups to determine the fertile and infertile phenotype to identify the genetic aspects step, amplified fragment length polymorphism (AFLP) method and a bulk separation analysis (BSA) navigating to use molecular markers associated with the phase, and switches to cut polymorphic amplified fragment (CAPS) molecular markers that can be easily applied in breeding programs that step, developing the amplified fragment polymorphism cut (CAPS) is characterized in that the male sterile genotype to determine molecular marker and by using the step and the selection technique to show how to cultivate a new male sterility system in a short period of time. ; skills on how to develop molecular markers linked to genetic male sterility, and to foster a new system from later generations of backcrossing, or it can be applied to all children using pepper using genetic male sterility, as well as paprika There. Molecular markers developed in this invention also may be applied to develop a new male sterility (GMS) system (the mother) of all introduced up to now paprika, separate groups to distinguish between heterozygous and homozygous male fertile of it later in Because there is no need to test as compared to existing conventional breeding methods according a breeding cost savings and reduce packaging consumption needs significantly breeding and breeding on light as well as low labor input can be reduced for very large male sterility system development It is expected.
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