首页> 外国专利> METHOD OF PROMOTING SUBSISTENCE AND/OR PROLIFERATION OF NEURAL STEM CELL AND PROMOTING EXTENSION OF NEURITE, PROMOTER THEREFOR, PHARMACEUTICAL COMPOSITION CONTAINING NEURAL STEM CELL, METHOD OF ASSAY AND METHOD OF SCREENING

METHOD OF PROMOTING SUBSISTENCE AND/OR PROLIFERATION OF NEURAL STEM CELL AND PROMOTING EXTENSION OF NEURITE, PROMOTER THEREFOR, PHARMACEUTICAL COMPOSITION CONTAINING NEURAL STEM CELL, METHOD OF ASSAY AND METHOD OF SCREENING

机译：促进神经干细胞的生存和/或繁殖和促进神经胶质细胞的延伸的方法，促进其的发展，包含神经干细胞的药物成分，测定方法和筛选方法

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PROBLEMS TO BE SOLVED Methods for enhancing survival and/or proliferation of neural stem cells and pharmaceutical compositions containing neural stem cells prepared by such methods, together with methods for assaying factors enhancing survival and/or proliferation of neural stem cells and methods for screening for such factors. MEANS FOR SOLVING THE PROBLEM Either Galectin-l is overexpressed in neural stem cells or neural stem cells are cultured in a liquid medium containing Galectin-1. Pharmaceutical compositions containing Galectin-1-overexpressing neural stem cells and pharmaceutical composition containing Galecti-n-1, prepared by the aforementioned methods, improve higher cerebral functions damaged by cerebral ischemia. Further, by seeding neural stem cells at clonal concentrations and determining whether the seeded neural stem cells are capable of proliferating in an assay medium to be assayed, whether the factor enhances survival and/or proliferation of neural stem cells is assayed and a factor enhancing survival and/or proliferation of neural stem cells are identified using this assay method.

机译：解决的问题增强神经干细胞的存活和/或增殖的方法以及通过这种方法制备的含有神经干细胞的药物组合物，以及测定增强神经干细胞的存活和/或增殖的因子的方法以及筛选此类方法的方法因素。解决问题的方法在神经干细胞中过表达Galectin-1或在含有Galectin-1的液体培养基中培养神经干细胞。通过上述方法制备的含有过表达Galectin-1的神经干细胞的药物组合物和含有Galecti-n-1的药物组合物可改善因脑缺血而受损的高级脑功能。此外，通过以克隆浓度接种神经干细胞并确定接种的神经干细胞是否能够在待测定的测定培养基中增殖，可以测定该因子是否增强了神经干细胞的存活和/或增殖，并且测定了是否增加了存活率使用该测定方法鉴定神经干细胞的增殖和/或增殖。

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公开/公告号EP1674566B1

专利类型
公开/公告日2011-04-13

原文格式PDF
申请/专利权人 KEIO UNIVERSITY;
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申请/专利号EP20040787726
发明设计人 OKANO HIDEYUKIKEIO UNIVERSITY SCHOOL OF MEDICINE;OKANO JAMES H.KEIO UNIVERSITY SCHOOL OF MEDICINE;SAKAGUCHI MASANORIKEIO UNIV. SCHOOL OF MEDICINE;MIZUSAWA H.DEPT. OF NEUROLOGY NEUROLOGICAL;ISHIBASHI S.DEPT. OF NEUROLOGY NEUROLOGICAL;
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申请日2004-09-08
分类号C12N15;A61K35/12;C12N5;A61L27;A61P9;A61P25;
国家 EP
入库时间 2022-08-21 17:59:15

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