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Method for detection of liver cancer, liver cancer risk, the risk of recurrence of liver cancer, liver cancer malignancy and progression in time of liver cancer based on the methylated cytosine of BASP1 gene.
Method for detection of liver cancer, liver cancer risk, the risk of recurrence of liver cancer, liver cancer malignancy and progression in time of liver cancer based on the methylated cytosine of BASP1 gene.
Method for detecting the presence and / or amount of methylated cytosines specific to liver cancer in a region containing CpG sequences in the BASP1 gene and optionally the SRD5A2 gene comprising the following steps (a) to (d): (a) isolating genomic DNA from a patient sample; (B) contacting a reagent for chemical or enzymatic treatment with genomic DNA to discriminate between methylated cytosines and unmethylated; (C) amplifying a region containing methylated cytosines of BASP1 gene and optionally the SRD5A2 gene in the genomic DNA using a PCR method; and (d) determining the presence and / or amount of methylated cytosine specific to liver cancer in a region containing CpG sequences of BASP1 gene and optionally the SRD5A2 gene in the sample of said patient, so that the presence and / or amount of cytosine specific methylated liver cancer indicate that the patient is afflicted with a liver cancer, has an increased risk of liver cancer, presents a risk of recurrence after treatment of liver cancer, it has a malignant liver cancer, or has a liver cancer which is progressing in time.
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