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SELECTION PROCEDURE FOR INTERNALIZING ANTIBODIES

机译：抗体国际化的选择程序

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摘要

This invention provides methods of selecting antibodies that are internalized into target cells. The methods generally involve contacting target cells with one or more members of an antibody phage display library. The members of the phage display library are also contacted with cells of a subtractive cell line. The target cells are then washed to remove the subtractive cell line cells and members of the phage display library that are non-specifically bound or weakly bound to the target cells. The target cells are cultured under conditions where members of the phage display library can be internalized if bound to an internalizing marker and internalized members of the phage display library are then identified.

机译：本发明提供了选择被内在化为靶细胞的抗体的方法。该方法通常包括使靶细胞与抗体噬菌体展示文库的一个或多个成员接触。噬菌体展示文库的成员也与减性细胞系的细胞接触。然后洗涤靶细胞以除去与靶细胞非特异性结合或弱结合的减性细胞系细胞和噬菌体展示文库成员。在结合至内在化标记物的情况下可将内化噬菌体展示库成员的条件下培养靶细胞，然后鉴定噬菌体展示库的内化成员。

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公开/公告号AT525385T

专利类型
公开/公告日2011-10-15

原文格式PDF
申请/专利权人 THE REGENTS OF THE UNIVERSITY OF CALIFORNIA;
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申请/专利号AT19990921396T
发明设计人 MARKS JAMES;POUL MARIE-ALIX;BECERRIL BALTAZAR;
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申请日1999-04-22
分类号C07H21/04;G01N33/68;A61K38;A61K39/395;A61K47/48;A61P37/02;C07K14/01;C07K16/28;C07K16/32;C12N7;C12N7/04;C12N15/09;C12N15/10;C12N15/62;C12N15/63;C12N15/86;C12Q1/70;C40B20/08;C40B30/04;C40B40/02;C40B50/06;G01N33/15;G01N33/50;G01N33/554;G01N33/566;
国家 AT
入库时间 2022-08-21 18:03:02

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