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method for making mycobacterial antigens for the differential serodiagnosis of tuberculosis in cattle

机译:牛结核分枝杆菌血清学诊断的分枝杆菌抗原的制备方法

摘要

A method for making mycobacterial antigens for the differential serodiagnosis of tuberculosis in cattle comprises the growth of Mycobacterium tuberculosis, bovine human and avian species, the inactivation of bacterial mass, release of antigens, their standardization, protein content. The bacterial mass Mycobacterium bovis, Mycobacterium tuberculosis, Mycobacterium avium grown in nutrient Saughton medium, autoclaved for 1 hour at 2 atm, washed and resuspended in sterile distilled water to concentration of 40 mg of bacterial mass in 1 cm3 of water, disintegration with ultrasound is performed at 22 kHz for 10-30 minutes followed by centrifugation at 1.5 thousand rev/min. for 20 minutes. The resulting supernatant is subjected to repeated sonification at 22 kHz for 10-30 minutes. After that antigens are standardized on protein content at spectrophotometer.
机译:一种用于牛的结核分枝杆菌血清学鉴别诊断的分枝杆菌抗原的制备方法,包括结核分枝杆菌,牛,人和禽类的生长,细菌质量的失活,抗原的释放,它们的标准化,蛋白质含量。细菌物质牛分枝杆菌,结核分枝杆菌,鸟分枝杆菌在营养的Saughton培养基中生长,在2 atm高压灭菌1小时,洗涤并重悬于无菌蒸馏水中,使其在1 cm3的水中浓缩为40 mg细菌,然后超声崩解。在22kHz下进行10-30分钟,然后以1.5千转/分钟离心。 20分钟。将所得的上清液在22kHz下重复超声处理10-30分钟。之后,在分光光度计上对抗原的蛋白质含量进行标准化。

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