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Method and kit for the extraction of DNA from Vitis vinifera L. and the amplificau00c7u00c3o and detection of vine varieties and varieties in musts or wines
Method and kit for the extraction of DNA from Vitis vinifera L. and the amplificau00c7u00c3o and detection of vine varieties and varieties in musts or wines
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机译：从葡萄和酒中提取DNA的方法和试剂盒，并检测葡萄汁和葡萄汁中的葡萄品种和品种
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摘要
This invenu00c7u00c3o describes a method for extraction of DNA from Vitis vinifera L., comprising the following steps: a.precipitar samples of must or wine with isopropanol or 2 propranolol, extract and collect the sediment; B.Incubate the sample resulting from the previous step with an extraction buffer that comprises: a solubilizador membrane of preference; one or more agents, anti oxidants, a chelator of divalent cations substance, Mg + 2 and / or Ca + 2; C. to carry out one or more of the sample resulting from the extracu00c7u00d5es The previous stage, using chloroform and / or phenol; D.To carry out one or more periods of precipitation and hatching with RNases; e. detecting the varieties or castas.descreve even a case of extraction of DNA in samples of must or wine comprising a solution containing the extraction buffer and the extraction is carried out according to The method described.Method and kit can be implemented as traceability system to ensure the origin of a product, detect fraud and misleading labelling, presenting as solution advantageous in allowing for the extraction of DNA from the vine from musts and wines single varietal and commercial. , enabling the detection and quantification of different varieties of vine in musts and wines.
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