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DNA EXTRACTION METHOD FOR BURSAPHELENCHUS XYLOPHILUS FROM WOOD CHIPS, LAMP PRIMER SET FOR BURSAPHELENCHUS XYLOPHILUS, AND DETECTION METHOD FOR BURSAPHELENCHUS XYLOPHILUS FROM WOOD CHIPS
DNA EXTRACTION METHOD FOR BURSAPHELENCHUS XYLOPHILUS FROM WOOD CHIPS, LAMP PRIMER SET FOR BURSAPHELENCHUS XYLOPHILUS, AND DETECTION METHOD FOR BURSAPHELENCHUS XYLOPHILUS FROM WOOD CHIPS
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机译:从木薯中提取黄花菜的DNA方法,木薯的初生灯组和从木薯中提取草菜的方法
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摘要
PROBLEM TO BE SOLVED: To provide a method for extracting and detecting Bursaphelenchus xylophilus DNA from a wood chip originated from a tree belonging to genus Pinus or the like without separating the Bursaphelenchus xylophilus.;SOLUTION: Provided are a method for extracting the DNA of the Bursaphelenchus xylophilus from a picked Bursaphelenchus xylophilus-containing wood chip; a LAMP primer set comprising a primer annealing the specific region of the DNA of the Bursaphelenchus xylophilus; and a method for detecting the Bursaphelenchus xylophilus from a wood chip, by amplifying the DNA by a LAMP method using the primer set.;COPYRIGHT: (C)2010,JPO&INPIT
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