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TISSUE-LIKE ORGANIZATION OF CELLS, MACROSCOPIC TISSUE-LIKE CONSTRUCTS GENERATED BY MACROMASS CULTURE OF CELLS AND THE METHOD OF MACROMASS CULTURE
TISSUE-LIKE ORGANIZATION OF CELLS, MACROSCOPIC TISSUE-LIKE CONSTRUCTS GENERATED BY MACROMASS CULTURE OF CELLS AND THE METHOD OF MACROMASS CULTURE
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机译:细胞的组织组织,细胞的宏观培养产生的宏观组织样结构和宏观培养方法
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摘要
Three-dimensional tissue-like organization of cells by high cell-seeding-density culture termed as macromass culture is described. By macromass culture, cells can be made to organize themselves into a tissue-like form without the aid of a scaffold and three-dimensional macroscopic tissue-like constructs can be made wholly from cells. Tissue-like organization and macroscopic tissue-like constructs can be generated from fibroblastic cells of mesenchymal origin (at least), which can be either differentiated cells or multipotent adult stem cells. In this work, tissue-like organization and macroscopic tissue-like constructs have been generated from dermal fibroblasts, adipose stromal cells-derived osteogenic cells, chondrocytes, and from osteoblasts. The factor causing macroscopic tissue formation is large scale culture at high cell seeding density per unit area or three-dimensional space, that is, macromass culture done on a large scale. No scaffold or extraneous matrix is used for tissue generation, the tissues are of completely cellular origin. No other agents (except high cell-seeding-density) that aid in tissue formation such as tissue-inducing chemicals, tissue-inducing growth factors, substratum with special properties, rotational culture, etc, are employed for tissue formation. These tissue-like masses have the potential for use as tissue replacements in the human body. Tissue-like organization by high cell-seeding-density macromass culture can also be generated at the microscopic level.
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