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Method for parallel isolation of double and single stranded nucleic acids and for selective removal of double stranded nucleic acids from a mixture of double and single stranded nucleic acids
Method for parallel isolation of double and single stranded nucleic acids and for selective removal of double stranded nucleic acids from a mixture of double and single stranded nucleic acids
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机译：平行分离双链和单链核酸并从双链和单链核酸的混合物中选择性除去双链核酸的方法
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摘要
A method for i) parallel isolation of a double-stranded and/or a single-stranded nucleic acid and/or ii) selective removal of a double-stranded nucleic acid from a mixture of a double-stranded and a single-stranded nucleic acid or from a source comprising a double-stranded and a single-stranded nucleic acid includes absorbing the double-stranded nucleic acid onto a first solid carrier, while the single-stranded nucleic acid is not adsorbed and remains in solution, removing the first carrier with the adsorbed nucleic acid from the solution, mixing the solution comprising the single-stranded nucleic acid with an alcoholic solution having a concentration of 1 to 90 vol.-%, and contacting the resulting solution with second solid carrier, to absorb the single-stranded nucleic acid onto the second solid carrier.
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