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competitive enzyme-linked immunosorbent assay (c-elisa) for the detection of a specific flavivirus antibody
competitive enzyme-linked immunosorbent assay (c-elisa) for the detection of a specific flavivirus antibody
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机译：竞争性酶联免疫吸附测定（c-elisa），用于检测特定的黄病毒抗体
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摘要
IMONOABSORVENT ASSAY CONNECTED TO COMPETITIVE ENZYME (C ELISA) FOR THE DETECTION OF A SPECIFIC FLAVIVIRUS ANTIBODY A competitive enzyme-linked immunosorbent assay (C-ELISA), using flavivirus member-specific immunological agents was developed to detect the specific antibody to members of flaviviruses. flaviviruses indicative of exposure to flaviviruses. The test is based on a competition for the binding epitope on the envelope protein of the flavivirus antigen captured using antiflavivirus IgA in the presence of positive flavivirus serum. This test has the sensitivity, specificity and speed comparable to the virus neutralization assay (VNT). ELISA is a versatile technique, which could have several applications. Slight modifications to this protocol could lead to an ELISA-based detection method of secondary infection or one that could be used for serotype-specific serotype and / or vaccine evaluation studies. The protocol developed for C-ELISA was demonstrated using the antigen of used dengue and the dengue specific monoclonal antibody. This can be used against other flaviviruses and the results for Japanese encephalitis illustrate this.
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