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thraustochytrid delta-6 desaturase and its uses

机译:破囊壶菌δ6去饱和酶及其用途

摘要

DELTA-6 THRAUSTOCHYTRID DESATURASE AND ITS USES. The present invention relates to a Delta-6 desaturase gene isolated from Schizochytríum. it is also directed to the cloning of Delta-6 desaturase derived from Schizochytrium in Yeast. The sequence of nucleic acids and the amino acid sequences of Delta-6 desaturase are described. Also described are the constructs, a vector comprising the gene encoding the Delta-6 desaturase enzyme in functional combination with the heterologous regulatory sequences. The new Delta-6 desaturase can be used in a metabolic pathway to convert linoleic acid to gamma linolenic acid (omega-6 pathway). The invention provides for the identification, isolation of these new Schizochytrium nucleic acids that encode the proteins mentioned above. The invention specifically exemplifies recombinant yeast cells harboring the vector comprising the Delta-6 desaturase gene and by virtue of the enzyme produced it may be able to produce gamma linolenic acid.
机译:DELTA-6硫杂缩醛饱和酶及其用途。本发明涉及从裂殖酵母分离的Delta-6去饱和酶基因。它也涉及酵母中裂殖壶菌衍生的Delta-6去饱和酶的克隆。描述了核酸序列和Delta-6去饱和酶的氨基酸序列。还描述了构建体,其是包含与异源调节序列功能性组合的编码Delta-6去饱和酶的基因的载体。新的Delta-6去饱和酶可用于新陈代谢途径,将亚油酸转化为伽马亚麻酸(ω-6途径)。本发明提供了鉴定,分离这些编码上述蛋白质的新的裂殖壶菌核酸。本发明具体地举例说明了带有包含Delta-6去饱和酶基因的载体的重组酵母细胞,并且由于产生的酶,它可能能够产生γ亚麻酸。

著录项

  • 公开/公告号BRPI0613685A2

    专利类型

  • 公开/公告日2011-01-25

    原文格式PDF

  • 申请/专利权人 AVESTHA GENGRAINE TECHNOLOGIES PVT LTD;

    申请/专利号BR2006PI13685

  • 发明设计人 VILLOO MORAWALA PATELL;

    申请日2006-07-20

  • 分类号C12N15/53;A61K38/43;C12N9/02;C12N15/81;C12P7/64;

  • 国家 BR

  • 入库时间 2022-08-21 18:07:17

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