首页> 外国专利> METHOD AND KIT FOR QUANTIFYING OF ALLERGEN-SPECIFIC HUMAN IGG SUBCLASSES FOR THE CONTROL AND ATTENDANCE OF SPECIFIC IMMUNOTHERAPY

METHOD AND KIT FOR QUANTIFYING OF ALLERGEN-SPECIFIC HUMAN IGG SUBCLASSES FOR THE CONTROL AND ATTENDANCE OF SPECIFIC IMMUNOTHERAPY

机译:用于量化和控制特异性免疫治疗的过敏原特异性人类Igg亚类的方法和工具包

摘要

A method for quantifying the levels of allergen-specific human IgG antibody subclasses, particularly of IgG1, IgG2, IgG3 or IgG4 subclasses, using a reverse ELISA immunoenzymatic technique. Also provided is a kit comprising microtitration plates for ELISA already sensitized/covered with monoclonal antibodies, for example, anti-Der p 1 or anti-Der p 2, total extract from corresponding allergens, labeled secondary antibodies, enzymatic conjugate, enzymatic substrate, and chromogenic buffer, as well as reference positive and negative control sera. The method provides high sensitivity for the detection of specific IgG antibody subclasses and is a potential tool for the control and attendance of allergen-specific immunotherapy in patients with allergic respiratory disease.
机译:一种使用反向ELISA免疫酶技术定量过敏原特异性人IgG抗体亚类(尤其是IgG1,IgG2,IgG3或IgG4亚类)水平的方法。还提供了一种试剂盒,其包括已经用单克隆抗体(例如,抗Der p 1或抗Der p 2)敏化/覆盖的用于ELISA的微量滴定板,其来自相应变应原的总提取物,标记的第二抗体,酶结合物,酶底物和生色缓冲液以及参考阳性和阴性对照血清。该方法为检测特定IgG抗体亚类提供了高灵敏度,并且是控制和治疗过敏性呼吸系统疾病患者的过敏原特异性免疫疗法的潜在工具。

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