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IDENTIFICATION OF BACTERIAL SPECIES AND SUBSPECIES USING LIPIDS

机译：使用脂质鉴定细菌种类和亚种

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摘要

The use of free, extractable lipids found in bacteria for identification of bacterial species and subspecies is described. Bacteria have been found to differ sufficiently in their extracted lipid compositions to effect identification using thin layer chromatographic techniques. Burkholderia pseudomallei, Burkholderia thailandensis, and Burkholderia mallei have been distinguished in this manner. Lipopeptides specific to Mycobacterium avium subspecies paratuberculosis, but not to the closely related bacterium Mycobacterium avium subspecies avium have also been used as a basis for bacterial subspecies identification using mass spectrometry and seroreactivity. Mass spectrometric analysis of total bacterial lipids of Burkholderia pseudomallei, Burkholderia thailandensis, and Burkholderia mallei, and mass spectrometric analysis of total bacterial lipids for Mycobacterium avium subspecies paratuberculosis and Mycobacterium avium subspecies avium, without further lipid separation, has shown that species and subspecies of bacteria may be identified using such analysis.

机译：描述了在细菌中发现的游离的，可提取的脂质用于鉴定细菌种类和亚种的用途。已经发现细菌在其提取的脂质组成上有足够的差异，以利用薄层色谱技术进行鉴定。以此方式区分了假伯克霍尔德氏菌，泰国伯克霍尔德氏菌和伯克霍尔德氏菌。还已经发现了对禽分枝杆菌亚种副结核病而不是与紧密相关的细菌禽分枝杆菌禽种特异的脂肽用作使用质谱和血清反应鉴定细菌亚种的基础。麻风伯克霍尔德氏菌，泰国伯克霍尔德氏菌和玛氏伯克霍尔德氏菌的总细菌脂质的质谱分析和鸟分枝杆菌的总细菌脂质的质谱分析在没有进一步分离脂质的情况下，副结核结核菌和禽分枝杆菌禽分枝杆菌表明，可以使用这种分析方法鉴定细菌的种类和亚种。 展开▼

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公开/公告号US2011086384A1

专利类型

公开/公告日2011-04-14

原文格式PDF

申请/专利权人 TORSTEN MANFRED ECKSTEIN;JULIA MITSUE INAMINE ECKSTEIN;
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申请/专利号US20100959248

发明设计人 TORSTEN MANFRED ECKSTEIN;JULIA MITSUE INAMINE ECKSTEIN;
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申请日2010-12-02

分类号C12Q1/04;

国家 US

入库时间 2022-08-21 18:15:27

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