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Polymerase chain reaction block and continuous real-time monitoring device using the same

机译:聚合酶链反应块和使用该反应块的连续实时监测装置

摘要

Disclosed is real-time monitoring apparatus comprising a thermal cycling reaction block having heating block which is formed of a hollow part and divided by an insulating layer, and a capillary tube through which a sample is flowed in and/or out and which is wound on the heating block so that the different temperatures are transferred and thus reaction cycle is repeatedly performed; a light source; a band pass filter; a condensing lens; a beam splitter; a reflecting mirror which is rotatably connected with a motor so as to transfer the excitation light reflected from the beam splitter to the capillary tube and reflect the fluorescence generated from the sample in the capillary tube; and a fluorescence detecting part.
机译:公开了一种实时监测装置,其包括:热循环反应块,其具有由中空部分形成并由绝缘层隔开的加热块;以及毛细管,通过该毛细管流入和/或流出样品,并缠绕在该毛细管上。加热块,以便传递不同的温度,从而重复进行反应循环。光源;带通滤波器;聚光镜;分束器;反射镜,其与电动机可旋转地连接,以将从分束器反射的激发光传输至毛细管,并反射从毛细管中的样品产生的荧光。荧光检测部。

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