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CULTURE SOLUTION FOR MEASURING MEMBRANE POTENTIAL OF CELL, CULTURE METHOD USING THE SAME, AND METHOD FOR MEASURING MEMBRANE POTENTIAL
CULTURE SOLUTION FOR MEASURING MEMBRANE POTENTIAL OF CELL, CULTURE METHOD USING THE SAME, AND METHOD FOR MEASURING MEMBRANE POTENTIAL
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机译:用于测量细胞膜电位的培养溶液,使用该方法的培养方法以及用于测量膜电位的方法
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摘要
PROBLEM TO BE SOLVED: To solve such a problem that interaction or adsorption frequently occurs among substances such as amino acids and proteins and a test compound for evaluating pharmaceutical effects, and the pharmaceutical effects cannot accurately be evaluated because a conventional culture solution contains large amounts of the substances.;SOLUTION: Accurate measurement can be carried out without inhibiting a fluorescent label and without causing the interaction or adsorption among the substances and the test compound for evaluating the pharmaceutical effects during a test for evaluating the pharmaceutical effects by including selenium and/or nitrate ions and iron ions without adding the amino acids and proteins in the culture solution for measuring the membrane potential of a cell. Furthermore, the membrane potential can be measured, particularly in a cardiomyocyte derived from a human induced pluripotent stem (iPS) cell over a long period of time by including the selenium and/or nitrate ions and iron ions in the culture solution.;COPYRIGHT: (C)2011,JPO&INPIT
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