首页> 外国专利> CULTURE SOLUTION FOR MEASURING MEMBRANE POTENTIAL OF CELL, CULTURE METHOD USING THE SAME, AND METHOD FOR MEASURING MEMBRANE POTENTIAL

CULTURE SOLUTION FOR MEASURING MEMBRANE POTENTIAL OF CELL, CULTURE METHOD USING THE SAME, AND METHOD FOR MEASURING MEMBRANE POTENTIAL

机译:用于测量细胞膜电位的培养溶液,使用该方法的培养方法以及用于测量膜电位的方法

摘要

PROBLEM TO BE SOLVED: To solve such a problem that interaction or adsorption frequently occurs among substances such as amino acids and proteins and a test compound for evaluating pharmaceutical effects, and the pharmaceutical effects cannot accurately be evaluated because a conventional culture solution contains large amounts of the substances.;SOLUTION: Accurate measurement can be carried out without inhibiting a fluorescent label and without causing the interaction or adsorption among the substances and the test compound for evaluating the pharmaceutical effects during a test for evaluating the pharmaceutical effects by including selenium and/or nitrate ions and iron ions without adding the amino acids and proteins in the culture solution for measuring the membrane potential of a cell. Furthermore, the membrane potential can be measured, particularly in a cardiomyocyte derived from a human induced pluripotent stem (iPS) cell over a long period of time by including the selenium and/or nitrate ions and iron ions in the culture solution.;COPYRIGHT: (C)2011,JPO&INPIT
机译:要解决的问题:为了解决这样的问题,即氨基酸和蛋白质等物质与用于评价药物作用的测试化合物之间经常发生相互作用或吸附,并且由于常规培养液中含有大量的药物,因此无法准确地评估药物作用解决方案:可以在不抑制荧光标记的情况下进行准确的测量,也不会引起物质和受试化合物之间的相互作用或吸附,从而在评估药物作用的测试过程中(包括硒和/或硒)硝酸盐离子和铁离子,而无需在培养液中添加用于测量细胞膜电位的氨基酸和蛋白质。此外,通过在培养液中加入硒和/或硝酸根离子和铁离子,可以长时间测量膜电位,尤其是在人类诱导多能干(iPS)细胞衍生的心肌细胞中。 (C)2011,日本特许厅&INPIT

著录项

  • 公开/公告号JP2011015643A

    专利类型

  • 公开/公告日2011-01-27

    原文格式PDF

  • 申请/专利权人 REPROCELL INC;

    申请/专利号JP20090162753

  • 发明设计人 SAITO TSUNAYOSHI;ASAI YASUYUKI;

    申请日2009-07-09

  • 分类号C12Q1/02;G01N27/416;

  • 国家 JP

  • 入库时间 2022-08-21 18:23:53

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