首页> 外国专利> Identifying species of Methylobacterium, comprises preparing crude extract from methylobacteria, amplifying 16S ribosomal RNA, carrying out restriction digest, analyzing restriction fragment, detecting restriction pattern and comparing

Identifying species of Methylobacterium, comprises preparing crude extract from methylobacteria, amplifying 16S ribosomal RNA, carrying out restriction digest, analyzing restriction fragment, detecting restriction pattern and comparing

机译：鉴定甲基细菌的种类，包括从甲基细菌制备粗提物，扩增16S核糖体RNA，进行限制性酶切，分析限制性片段，检测限制性酶切图谱并进行比较

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摘要

Identifying different species of genus Methylobacterium, comprises: (a) preparing a crude extract from suspension of methylobacteria; (b) amplifying 16S rRNA (rRNA) gene by PCR; (c) carrying out at least two restriction digests of the PCR products; (d) separating and analyzing the restriction fragments of two restriction digests; (e) detecting at least two restriction patterns of the parallelly arranged restriction digest; and (f) comparing the restriction patterns and DNA fragment length with the known restriction patterns of reference strains of Methylobacterium. Identifying different species of genus Methylobacterium, comprises: (a) preparing a crude extract from a suspension of methylobacteria; (b) amplifying 16S rRNA gene by PCR using the crude extract as DNA matrix and the oligonucleotides pA and pH as primers; (c) carrying out at least two restriction digests of the PCR products in parallel reaction batches; (d) separating and analyzing the restriction fragments of at least two restriction digests by gel electrophoresis; (e) detecting at least two restriction patterns of the parallelly arranged restriction digest; and (f) comparing the restriction patterns and the DNA fragment length with the known restriction patterns of reference strains of Methylobacteriumfor the identification of the methylobacteria strain in the crude extract.

机译：鉴定甲基杆菌属的不同种类包括：（a）从甲基杆菌悬液中制备粗提物; （b）通过PCR扩增16S rRNA（rRNA）基因; （c）对PCR产物进行至少两种限制性消化; （d）分离和分析两个限制性消化的限制性片段; （e）检测平行排列的限制摘要的至少两个限制模式; （f）将限制性酶切图谱和DNA片段长度与甲基杆菌参考菌株的已知限制性酶切图谱进行比较。鉴定甲基杆菌属的不同种类包括：（a）从甲基杆菌悬液中制备粗提物; （b）以粗提取物为DNA基质，以寡核苷酸pA和pH为引物，通过PCR扩增16S rRNA基因; （c）以平行反应批次进行至少两次PCR产物的限制性消化; （d）通过凝胶电泳分离和分析至少两个限制性消化的限制性片段; （e）检测平行排列的限制摘要的至少两个限制模式; （f）将限制性图谱和DNA片段长度与甲基杆菌参考菌株的已知限制性图谱进行比较，以鉴定粗提物中的甲基细菌菌株。

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公开/公告号DE102008002978A1

专利类型
公开/公告日2009-12-10

原文格式PDF
申请/专利权人 UNIVERSITAET KASSEL;
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申请/专利号DE20081002978
发明设计人 KROLCZYK SABINE;
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申请日2008-07-31
分类号C12Q1/02;
国家 DE
入库时间 2022-08-21 18:29:01

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