首页> 外国专利> Isolation of urate oxidase, urate oxidase purified chimeric AND ITS CONJUGATES, purified fragment urate oxidase, urate oxidase CONJUGATES OF PURIFIED (VARIANTS) PHARMACEUTICAL COMPOSITION BASED ON CONJUGATE OF PURIFIED urate oxidase, urate oxidase AND METHOD FOR CLEANING Dedicated urate oxidase produced by this process

Isolation of urate oxidase, urate oxidase purified chimeric AND ITS CONJUGATES, purified fragment urate oxidase, urate oxidase CONJUGATES OF PURIFIED (VARIANTS) PHARMACEUTICAL COMPOSITION BASED ON CONJUGATE OF PURIFIED urate oxidase, urate oxidase AND METHOD FOR CLEANING Dedicated urate oxidase produced by this process

机译：尿酸盐氧化酶的分离，尿酸盐氧化酶纯化的嵌合体及其缀合物，纯化的片段尿酸盐氧化酶，尿酸盐氧化酶基于纯化尿酸盐氧化酶共轭的制药成分组成的变体，尿酸盐氧化酶及其制备方法

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1. Isolated uratoxidase (uricase), characterized in that at least 95% of the total number of its molecules is part of tetramers and octamers. ! 2. Uratoxidase according to claim 1, characterized in that the source from which it is isolated is a mammalian organism. ! 3. Uratoxidase according to claim 2, characterized in that it is isolated from the liver of a pig or the liver of a sheep. ! 4. Uratoxidase according to claim 1, characterized in that it is recombinant. ! 5. Uratoxidase according to claim 4, characterized in that it has the amino acid sequence of porcine liver uricase, bovine liver uricase, sheep liver uricase or baboon liver uricase. ! 6. Uratoxidase according to claim 4, characterized in that it is chimeric. ! 7. Uratoxidase according to claim 6, characterized in that it includes a portion of uricase isolated from pig liver and a portion of uricase isolated from baboon liver. ! 8. The uratoxidase according to claim 7, characterized in that it has the amino acid sequence of porcine uricase SEQ ID NO: 1 with a lysine residue instead of an arginine residue at position 291 (R291K) and with a serine residue instead of a threonine residue at position 301 (T301S) (CKS uricase). ! 9. Uratoxidase according to claim 4, characterized in that it has the amino acid sequence of SEQ ID NO: 2 with a histidine residue instead of tyrosine at position 97 (Y97H). ! 10. Uratoxidase according to claim 1, characterized in that the source from which it is isolated is an organism of a fungus or microbe. ! 11. The uratoxidase of claim 10, characterized in that it is isolated or recombinant and, moreover, has the amino acid sequence of an organism of a fungus or microbe selected from the group consisting of Aspergillus flavus, Arthrobacter globiformis, Bacillus sp. and Candida utilis. ! 12. Uratok

机译：1.分离的铀酰氧化酶（尿酸酶），其特征在于其分子总数的至少95％是四聚体和八聚体的一部分。！ 2.根据权利要求1的尿素过氧化物酶，其特征在于从其分离出的来源是哺乳动物生物体。！ 3.根据权利要求2的尿素过氧化物酶，其特征在于它是从猪的肝脏或绵羊的肝脏分离的。！ 4.根据权利要求1的尿素氧化酶，其特征在于它是重组的。！ 5.根据权利要求4的尿过氧化物酶，其特征在于其具有猪肝尿素酶，牛肝尿素酶，绵羊肝尿素酶或狒狒肝尿素酶的氨基酸序列。！ 6.根据权利要求4的尿素过氧化物酶，其特征在于它是嵌合的。！ 7.根据权利要求6的尿素过氧化物酶，其特征在于它包括从猪肝中分离出的一部分尿酸酶和从狒狒肝中分离出的一部分尿酸酶。！ 8.根据权利要求7的尿氧化酶，其特征在于它具有猪尿酸酶SEQ ID NO：1的氨基酸序列，在291位具有赖氨酸残基而不是精氨酸残基（R291K），并且具有丝氨酸残基而不是苏氨酸。 301位（T301S）残留（CKS尿酸酶）。！ 9.根据权利要求4的尿素过氧化物酶，其特征在于它具有SEQ ID NO：2的氨基酸序列，在97位（Y97H）具有组氨酸残基而不是酪氨酸。！ 10.根据权利要求1所述的脲氧化酶，其特征在于，从其分离出的来源是真菌或微生物。！ 11.权利要求10的尿氧化酶，其特征在于它是分离的或重组的，并且具有选自黄曲霉，球状节杆菌，芽孢杆菌属的真菌或微生物的氨基酸序列。和util假丝酵母。！ 12.浦生市

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公开/公告号RU2009104003A

专利类型
公开/公告日2010-08-20

原文格式PDF
申请/专利权人 МУНТЭН ВЬЮ ФАРМАСЬЮТИКЭЛЗ ИНК. (US);ДЮК ЮНИВЕРСИТИ (US);
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申请/专利号RU20090104003
发明设计人 ВИЛЛИАМЗ Л. ДЭВИД (US);КЕЛЛИ СЬЮЗЭН ДЖ. (US);САЙФЕР МАРК ДЖ. П. (US);ХЕРШФИЛД МАЙКЛ С. (US);ШЕРМЕН МЕРИ Р. (US);
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申请日2009-02-06
分类号C12N9/02;C12N15/53;C12N15/11;
国家 RU
入库时间 2022-08-21 18:30:02

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