首页> 外国专利> METHOD FOR DIFFERENTIATING HUMAN EMBRYONIC STEM CELL TO CARDIAC PROGENITOR CELLS OR CARDIAC MYOCYTE USING TREATMENT OF GAMMA SECRETASE INHIBITOR AND VOLUMINAL CONTROL OF CULTURE SOLUTION

METHOD FOR DIFFERENTIATING HUMAN EMBRYONIC STEM CELL TO CARDIAC PROGENITOR CELLS OR CARDIAC MYOCYTE USING TREATMENT OF GAMMA SECRETASE INHIBITOR AND VOLUMINAL CONTROL OF CULTURE SOLUTION

机译:通过γ分泌酶抑制剂的处理和培养液的体积控制将人胚胎干细胞分化为心脏祖细胞或心肌细胞的方法

摘要

The present invention relates to a method for promoting differentiation of human embryonic stem cells into myocardial precursors or cardiomyocytes using a gamma secretase inhibitor treatment and volume control of the culture medium, and more specifically, an agent for culturing human embryonic stem cells in an undifferentiated state. Stage 1; And a second step of treating the undifferentiated human embryonic stem cells with a gamma secretase inhibitor and differentiating them in a reduced volume of culture medium, unlike conventional differentiation methods, without having to go through the embryonic body formation step. The present invention relates to a method for promoting differentiation of human embryonic stem cells into myocardial precursors or cardiomyocytes, which can significantly shorten the differentiation period of the furnace and improve its differentiation efficiency.;Human Embryonic Stem Cells, Gamma Secretase Inhibitors, Myocardium, Differentiation
机译:本发明涉及一种使用γ分泌酶抑制剂处理和培养基的体积控制来促进人类胚胎干细胞向心肌前体或心肌细胞分化的方法,更具体地,涉及用于培养未分化状态的人类胚胎干细胞的试剂。 。阶段1;第二步是使用伽玛分泌酶抑制剂处理未分化的人类胚胎干细胞,然后在减少体积的培养基中分化它们,这与传统的分化方法不同,而无需经过胚胎体形成步骤。本发明涉及一种促进人胚胎干细胞向心肌前体或心肌细胞分化的方法,可以显着缩短炉子的分化周期,提高其分化效率。

著录项

  • 公开/公告号KR100958921B1

    专利类型

  • 公开/公告日2010-05-19

    原文格式PDF

  • 申请/专利权人

    申请/专利号KR20070110250

  • 发明设计人 최영민;장지호;문신용;오선경;

    申请日2007-10-31

  • 分类号C12N5/071;C12N5/02;C12N5;

  • 国家 KR

  • 入库时间 2022-08-21 18:31:13

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