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QUANTITATIVE ANALYSIS METHOD OF NARGENICIN BY A HIGH PERFORMANCE LIQUID CHROMATOGRAPHY CAPABLE OF QUANTIFYING AN ANTIBIOTIC MATERIAL IN A CELL-FREE CULTURE MEDIUM
QUANTITATIVE ANALYSIS METHOD OF NARGENICIN BY A HIGH PERFORMANCE LIQUID CHROMATOGRAPHY CAPABLE OF QUANTIFYING AN ANTIBIOTIC MATERIAL IN A CELL-FREE CULTURE MEDIUM
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机译：高效液相色谱法定量无细胞培养液中抗菌药物中降钙素的定量分析方法
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摘要
PURPOSE: A quantitative analysis method of nargenicin is provided to rapidly and accurately analyze the amount of the nargenicin in a cell-free culture medium, and to apply the method for analyzing other bacterial culture materials.;CONSTITUTION: A quantitative analysis method of nargenicin contained in Nocardia sp. CS682 cultures by a high performance liquid chromatography comprises the following steps: cultivating Nocardia species microorganisms and extracting the nargenicin from a culture broth using ethylacetate; detecting the nargenicin using a solvent a containing dipotassium phosphate and water, and a solvent B containing acetonitrile; forming the standard calibration curve of the nargenicin by plotting a concentration per peak region of the nargenicin; and quantitative-analyzing the nargenicin.;COPYRIGHT KIPO 2010
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