首页>
外国专利>
RECOMBINANT VECTOR FOR A DEVELOPMENT/SECRETION OF A FOREIGN PROTEIN CONTAINING A SIGNAL PEPTIDE, A PROTEIN DEVELOPMENT VERIFIER EPITOPE AND AN INSERTION SITE FOR THE FOREIGN PROTEIN
RECOMBINANT VECTOR FOR A DEVELOPMENT/SECRETION OF A FOREIGN PROTEIN CONTAINING A SIGNAL PEPTIDE, A PROTEIN DEVELOPMENT VERIFIER EPITOPE AND AN INSERTION SITE FOR THE FOREIGN PROTEIN
展开▼
机译:包含信号肽的外源蛋白,蛋白发育验证表位和外源蛋白插入位点的重组/分泌的重组载体
展开▼
页面导航
摘要
著录项
相似文献
摘要
PURPOSE: A recombinant vector for a development/secretion of a foreign protein is provided to improve the secretion efficiency and to confirm the development of the foreign protein by combining and epitope.;CONSTITUTION: A recombinant vector for a development/secretion of a foreign protein contains a signal peptide, a protein development verifier epitope and an insertion site for the foreign protein. The signal peptide is either an OmpW signal peptide, a StfA signal peptide or a AgfA signal peptide. The protein development verifier epitope is either a His epitope, an RGS-His epitope, a T7 epitope or a V5 epitope. The insertion site for the foreign protein comprises an EcoRI, BamHI, SalI and HindIII cutting region. The producing method of the foreign protein comprises the following steps: culturing a transformant obtained by transforming a host cell using the recombinant vector; and confirming the foreign protein at a western blot.;COPYRIGHT KIPO 2010
展开▼